Regulation of the mitogen-activated protein kinase p44 ERK activity during anoxia/recovery in rainbow trout hypodermal fibroblasts

The Journal of Experimental Biology
Carlo G OssumElse K Hoffmann

Abstract

It is well known from various mammalian cells that anoxia has a major impact on the mitogen-activated protein kinase ERK, but a possible similar effect in fish cells has not been investigated. Here we characterise a p44ERK-like protein in the rainbow trout cell line RTHDF and study the effect of (i) serum stimulation, (ii) sodium azide (chemical anoxia) and removal of azide (recovery) and (iii) anoxia (P(O)2<0.1%) and recovery. During both chemical and true anoxia p44ERK was inhibited and recovery resulted in robust reactivation of p44ERK activity, far above the initial level. The inhibition was secondary to activation of p38(MAPK) and the increase was MEK dependent, as SB203580 inhibited the dephosphorylation during anoxia and the presence of PD98059 inhibited phosphorylation of p44ERK during recovery. In addition, we demonstrated that the reactivation of p44ERK during recovery also was dependent on reactive oxygen species and a PP1/PP2A-like phosphatase.

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Citations

May 11, 2013·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Chang-Dong ZhuBiao Yan
Feb 2, 2008·Proteomics·Tune WulffElse K Hoffmann
Dec 10, 2009·International Journal of Cancer. Journal International Du Cancer·Lili LiuDaiming Fan
Nov 28, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Melissa R JunttilaJukka Westermarck
Nov 8, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Göran E NilssonKåre-Olav Stensløkken

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