Regulation of the mouse Treacher Collins syndrome homolog (Tcof1) promoter through differential repression of constitutive expression.

DNA and Cell Biology
Kathryn H Shows, Rita Shiang


Treacher Collins syndrome is an autosomal-dominant mandibulofacial dysostosis caused by haploinsufficiency of the TCOF1 gene product treacle. Mouse Tcof1 protein is approximately 61% identical and 71% similar to treacle, and heterozygous knockout of Tcof1 causes craniofacial malformation. Tcof1 expression is high in developing neural crest, but much lower in other tissues. To investigate this dual regulation, highly conserved regions upstream of TCOF1 homologs were tested through deletion and mutation reporter assays, and conserved predicted transcription factor binding sites were assessed through chromatin binding studies. Assays were performed in mouse P19 embryonic carcinoma cells and in HEK293 cells to determine differential activation in cell types at different stages of differentiation. Binding of Cebpb, Zfp161, and Sp1 transcription factors was specific to the Tcof1 regulatory region in P19 cells. The Zfp161 binding site demonstrated P19 cell-specific repression, while the Sp1/Sp3 candidate site demonstrated HEK293 cell-specific activation. Moreover, presence of c-myb and Zfp161 transcripts was specific to P19 cells. A minimal promoter fragment from -253 to +43 bp directs constitutive expression in both cell types, and d...Continue Reading


Mar 15, 1996·Nucleic Acids Research·M A HoeppnerB Wu
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·C A WiseE W Jabs
May 2, 1997·Biochimica Et Biophysica Acta·K SugiuraM Hagiwara
Sep 23, 1997·Biochemical and Biophysical Research Communications·W A PaznekasE W Jabs
May 7, 1998·The International Journal of Biochemistry & Cell Biology·L LaniaP De Luca
Mar 2, 1999·Biochemical and Biophysical Research Communications·T ObataS Yamamoto
Mar 19, 1999·Biochemical and Biophysical Research Communications·M NumotoJ Koshi
May 26, 1999·FEMS Microbiology Letters·T A Tatusova, T L Madden
Jun 27, 2000·Advances in Oto-rhino-laryngology·K L Marsh, M J Dixon
Sep 12, 2000·Molecular Biology of the Cell·C IsaacU T Meier
Sep 26, 2000·Molecular and Cellular Biology·D W O'NeillA Bank
Jul 27, 2001·Mammalian Genome : Official Journal of the International Mammalian Genome Society·K E HaworthY Edwards
Jan 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Rocio A LopezArthur Bank
May 15, 2002·The Biochemical Journal·Dipak P Ramji, Pelagia Foka
Jun 5, 2002·Genome Research·W James KentDavid Haussler
Jan 4, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ting LuoThomas D Sargent
Sep 16, 2003·Blood Cells, Molecules & Diseases·Scott A Ness
Nov 5, 2003·American Journal of Medical Genetics. Part a·Bozena MarszalekWieslaw H Trzeciak
Mar 26, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jill Dixon, Michael James Dixon
Jul 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Benigno C ValdezMichael J Dixon
Aug 25, 2004·The Journal of Biological Chemistry·Matthew J MorganJean Paul Thiery
Sep 2, 2004·European Journal of Human Genetics : EJHG·Ozge Altug TeberDagmar Wieczorek
Nov 4, 2004·Biochemical and Biophysical Research Communications·Michael MogassRita Shiang
Dec 4, 2004·Birth Defects Research. Part A, Clinical and Molecular Teratology·Partha MukhopadhyayM Michele Pisano
Jan 5, 2005·Biochemical and Biophysical Research Communications·Jun WangNobuyoshi Shimizu
Jun 3, 2005·Human Molecular Genetics·Bianca GonzalesBenigno C Valdez
Sep 28, 2005·Birth Defects Research. Part C, Embryo Today : Reviews·Véronique Lefebvre, Patrick Smits
Feb 9, 2006·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Kathryn H ShowsRita Shiang
Aug 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jill DixonPaul A Trainor
Dec 13, 2007·BioTechniques·H HolemonJ A Jeddeloh

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.