Regulation of the N-acyl homoserine lactone-dependent quorum-sensing system in rhizosphere Pseudomonas putida WCS358 and cross-talk with the stationary-phase RpoS sigma factor and the global regulator GacA

Applied and Environmental Microbiology
Iris Bertani, Vittorio Venturi

Abstract

Quorum sensing is a cell population-density dependent regulatory system which in gram-negative bacteria often involves the production and detection of N-acyl homoserine lactones (AHLs). Some Pseudomonas putida strains have been reported to produce AHLs, and one quorum-sensing locus has been identified. However, it appears that the majority of strains do not produce AHLs. In this study we report the identification and regulation of the AHL-dependent system of rhizosphere P. putida WCS358. This system is identical to the recently identified system of P. putida strain IsoF and very similar to the las system of Pseudomonas aeruginosa. It is composed of three genes, the luxI family member ppuI, the putative repressor rsaL, and the luxR family member ppuR. A genomic ppuR::Tn5 mutant of strain WCS358 was identified by its inability to produce AHLs when it was cross-streaked in close proximity to an AHL biosensor, whereas an rsaL::Tn5 genomic mutant was identified by its ability to overproduce AHL molecules. Using transcriptional promoter fusions, we studied expression profiles of the rsaL, ppuI, and ppuR promoters in various genetic backgrounds. At the onset of the stationary phase, the autoinducer synthase ppuI gene expression is und...Continue Reading

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D H Figurski, D R Helinski
Jan 1, 1982·Journal of Bacteriology·D CorbinD R Helinski
Jan 1, 1983·Methods in Enzymology·H C Birnboim
Mar 27, 1999·Journal of Bacteriology·T de KievitB H Iglewski
Jul 14, 2000·Journal of Bacteriology·M WhiteleyE P Greenberg
Jan 5, 2002·Molecular Plant-microbe Interactions : MPMI·S Heeb, D Haas
Mar 27, 2002·Journal of Bacteriology·Milan KojicVittorio Venturi
Mar 5, 2003·Current Opinion in Microbiology·Roger S Smith, Barbara H Iglewski
Oct 18, 2003·Journal of Bacteriology·Claudio AguilarVittorio Venturi
Feb 7, 2004·Molecular Microbiology·Martin SchusterE P Greenberg
Jan 1, 1987·Plant Molecular Biology·H P SpainkB J Lugtenberg

❮ Previous
Next ❯

Citations

Jun 26, 2007·Antonie van Leeuwenhoek·Jesús Mercado-Blanco, Peter A H M Bakker
Nov 25, 2011·Applied and Environmental Microbiology·Giordano RampioniLivia Leoni
Oct 30, 2007·Journal of Bacteriology·Brandan Hillerich, Janet Westpheling
Dec 31, 2005·Journal of Bacteriology·Giordano RampioniLivia Leoni
Jun 19, 2009·BMC Microbiology·Sujatha Subramoni, Vittorio Venturi
Jan 1, 2012·ISRN Microbiology·Zsolt GelencsérSándor Pongor
Nov 28, 2013·Antonie van Leeuwenhoek·Neera GargAditya Kumar
Apr 9, 2013·Applied Microbiology and Biotechnology·Gwynneth Felicity MatcherRosemary Ann Dorrington
Feb 5, 2016·Indian Journal of Microbiology·Shikha KoulVipin Chandra Kalia
Sep 25, 2014·Protein & Cell·Jasmine Lee, Lianhui Zhang
Apr 19, 2008·Journal of Microbiological Methods·Laura SteindlerVittorio Venturi
Nov 9, 2011·Molecular Microbiology·Vittorio VenturiLivia Leoni
Mar 7, 2014·Environmental Microbiology·Mustafa FazliTim Tolker-Nielsen
Feb 14, 2006·FEMS Microbiology Reviews·Vittorio Venturi
Dec 12, 2007·FEMS Microbiology Reviews·Eric PotvinRoger C Levesque
Oct 27, 2006·FEMS Microbiology Ecology·Ludovic VialFlorence Wisniewski-Dyé
Feb 25, 2010·Natural Product Reports·Jeroen S Dickschat
Mar 20, 2010·Genetika·M A Veselova

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.