PMID: 9654363Jul 8, 1998Paper

Regulation of the phosphorylation of glial fibrillary acidic protein (GFAP) by glutamate and calcium ions in slices of immature rat spinal cord: comparison with immature hippocampus

Neuroscience Letters
T KommersR Rodnight

Abstract

The effect of glutamate and lack of external Ca2+ on the phosphorylation of the astrocyte cell marker glial fibrillary acidic protein (GFAP) was studied in slices of hippocampus and thoracic spinal cord from immature (P12-16) rats. Confirming previous work with immature hippocampal slices (Wofchuk, S.T. and Rodnight, R., Neurochem. Int., 24 (1994) 517-523; Wofchuk, S.T. and Rodnight, R., Dev. Brain Res., 85 (1995) 181-186), glutamate strongly stimulated GFAP phosphorylation in media with Ca2+ and in media lacking Ca2+ a quantitatively similar stimulation of basal GFAP phosphorylation was observed. By contrast in slices of immature thoracic spinal cord, glutamate had no effect on GFAP phosphorylation and in media lacking Ca2+ phosphorylation of GFAP was inhibited. Since GFAP phosphorylation is Ca2+-dependent and is not stimulated by glutamate in slices of adult hippocampus, the present results suggest that astrocytic functions in the rat spinal cord mature more rapidly than in the hippocampus. The possibility that the difference in the control of GFAP phosphorylation in the two structures is related to differences in the control of GFAP dephosphorylation was investigated by incubating spinal cord slices with the calcineurin inhi...Continue Reading

References

Aug 18, 1991·The Journal of Comparative Neurology·E L GustafsonP Greengard
Mar 1, 1997·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·R RodnightR Leal

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Citations

May 16, 2007·Journal of Molecular Histology·Stanislava Jergová, Dása Cízková
Jul 31, 2001·Trends in Neurosciences·L R WatkinsS F Maier
May 22, 2002·Neurochemistry International·I SuárezB Fernández
Mar 8, 2005·Journal of Neurochemistry·Camilla I SvenssonTony L Yaksh
Jul 14, 2006·Experimental Gerontology·Robert G StrubleMary McAsey

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