Regulation of the rat liver sodium-dependent bile acid cotransporter gene by prolactin. Mediation of transcriptional activation by Stat5

The Journal of Clinical Investigation
T C GangulyM Vore

Abstract

The intracellular mechanism(s) underlying the upregulation of the hepatic Na+/taurocholate cotransporting polypeptide (ntcp) by prolactin (PRL) are unknown. In this report, we demonstrate a time-dependent increase in nuclear translocation of phosphorylated liver Stat5 (a member of the ignal ransducers and ctivators of ranscription family) that correlated with suckling-induced increases in serum PRL levels. In electrophoretic mobility gel shift assays, nuclear Stat5 exhibited specific DNA-binding ability towards IFN-gamma-activated sequence (GAS)-like elements (GLEs; 5'TTC/A-PyNPu-G/TAA-3') located in the -937 to -904 bp region of the ntcp promoter. Transient cotransfections in HepG2 cells revealed that PRL inducibility (2.5-3-fold) required coexpression of the long form of the PRL receptor (PRLRL) and Stat5. Deletion analysis mapped the PRLinducible region to -1237 to -758 bp of the ntcp promoter. Linking this 0.5-kb region to a heterologous thymidine kinase (tk) promoter, or linking multimerized ntcp GLEs either upstream of the ntcp minimal promoter (-158 to +47 bp) or the heterologous promoter conferred dose-dependent PRL responsiveness. The short form of the PRL receptor failed to transactivate ntcp GLEs. These results indic...Continue Reading

References

Mar 1, 1976·Metabolism: Clinical and Experimental·A C HeringtonW H Daughaday
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M MurakamiT Kishimoto
Sep 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M H Nathanson, J L Boyer
Oct 1, 1991·Proceedings of the Society for Experimental Biology and Medicine·R R Gala
Aug 1, 1991·Endocrine Reviews·P A KellyM Edery
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·B HagenbuchP J Meier
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L LesueurJ Djiane
Aug 1, 1990·Molecular Endocrinology·M ShirotaP A Kelly
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·G S CampbellC Carter-Su
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·J J LebrunP A Kelly
Mar 1, 1993·Cytokine·D Cosman
Oct 1, 1995·Current Opinion in Genetics & Development·B Groner, F Gouilleux
Jun 1, 1996·Molecular Endocrinology·E D GalsgaardN Billestrup

❮ Previous
Next ❯

Citations

Jul 14, 1999·Journal of Biochemical and Molecular Toxicology·J Kapitulnik, H W Strobel
Sep 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M ArreseF J Suchy
Nov 26, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A W WolkoffB F Scharschmidt
Apr 4, 2007·Pharmaceutical Research·Waddah A Alrefai, Ravinder K Gill
Mar 26, 1998·Journal of Hepatology·M Müller, P L Jansen
Mar 23, 2000·Journal of Hepatology·G A Kullak-UblickG Paumgartner
Jan 28, 2003·Journal of Hepatology·Marco ArreseFrederick J Suchy
Sep 17, 1999·Journal of Hepatology·G A Kullak-Ublick
Jan 12, 2000·Molecular and Cellular Endocrinology·D Devost, J M Boutin
Aug 28, 1998·Current Opinion in Cell Biology·B Stieger, P J Meier
Apr 15, 2008·Trends in Endocrinology and Metabolism : TEM·Z Z HuM L Dufau
Sep 13, 2000·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·H K Choi, D J Waxman
Apr 1, 2000·Cytokine & Growth Factor Reviews·P M GrimleyH Rui
Oct 7, 2006·Current Opinion in Gastroenterology·M Trauner, J L Boyer
Jun 30, 1998·Current Opinion in Lipidology·M W Love, P A Dawson
Apr 21, 2005·Molecular Pharmacology·M WoodM Vore
Feb 5, 2002·Annual Review of Physiology·Peter J Meier, B Stieger
Feb 23, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Clavia Ruth Wooton-KeeMary Vore
Jan 24, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Julia Yue CuiCurtis D Klaassen
Jun 6, 2009·Journal of Lipid Research·Paul A DawsonAnuradha Rao
Apr 7, 2010·Expert Opinion on Drug Metabolism & Toxicology·Hyunyoung Jeong
Feb 18, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Leander BlaasEmilio Casanova
Dec 4, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Clavia Ruth Wooton-KeeMary Vore
Mar 10, 2001·Clinics in Liver Disease·G A Kullak-Ublick, P J Meier
Jan 5, 2011·Biochimica Et Biophysica Acta·Thierry ClaudelMichael Trauner
Dec 18, 2013·Molecular Aspects of Medicine·Anna BaghdasaryanMichael Trauner
Mar 5, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M Sawkat Anwer
Mar 18, 2004·American Journal of Physiology. Endocrinology and Metabolism·Manuela MatasconiMats Rudling
Sep 14, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Francis R SimonEileen Sutherland
May 11, 2001·Molecular Carcinogenesis·G G OakleyL W Robertson
Jul 20, 2007·Fundamental & Clinical Pharmacology·Vera Dias, Vera Ribeiro
Jul 21, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Christoph G DietrichAndreas Geier
Aug 1, 2008·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·A Kosters, S J Karpen
Dec 10, 1999·The Journal of Biological Chemistry·P A Ram, D J Waxman
Jan 13, 2001·The Journal of Biological Chemistry·P M GowriM Vore

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.