Regulation of the rplY gene encoding 5S rRNA binding protein L25 in Escherichia coli and related bacteria

RNA
L V AseevIrina V Boni

Abstract

Ribosomal protein (r-protein) L25 is one of the three r-proteins (L25, L5, L18) that interact with 5S rRNA in eubacteria. Specific binding of L25 with a certain domain of 5S r-RNA, a so-called loop E, has been studied in detail, but information about regulation of L25 synthesis has remained totally lacking. In contrast to the rplE (L5) and rplR (L18) genes that belong to the polycistronic spc-operon and are regulated at the translation level by r-protein S8, the rplY (L25) gene forms an independent transcription unit. The main goal of this work was to study the regulation of the rplY expression in vivo. We show that the rplY promoter is down-regulated by ppGpp and its cofactor DksA in response to amino acid starvation. At the level of translation, the rplY expression is subjected to the negative feedback control. The 5'-untranslated region of the rplY mRNA comprises specific sequence/structure features, including an atypical SD-like sequence, which are highly conserved in a subset of gamma-proteobacterial families. Despite the lack of a canonical SD element, the rplY'-'lacZ single-copy reporter showed unusually high translation efficiency. Expression of the rplY gene in trans decreased the translation yield, indicating the mech...Continue Reading

References

Jan 11, 1991·Nucleic Acids Research·I V BoniN V Tzareva
Mar 11, 1987·Nucleic Acids Research·C B Harley, R P Reynolds
Jan 1, 1984·Annual Review of Biochemistry·M NomuraG Baughman
Mar 26, 1984·Nucleic Acids Research·A A Travers
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M NomuraL E Post
Jan 1, 1994·Progress in Nucleic Acid Research and Molecular Biology·J M Zengel, L Lindahl
Nov 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·A A BogdanovI N Lavrik
Mar 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·M Lu, T A Steitz
Feb 5, 2003·The Biochemical Journal·Maciej SzymańskiJan Barciszewski
May 20, 2003·The Journal of Biological Chemistry·Kirsty SalmonRobert P Gunsalus
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Aug 20, 2004·Journal of Bacteriology·Todd D AllenJanice M Zengel
May 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Brian J PaulRichard L Gourse
Nov 1, 2005·Critical Reviews in Biochemistry and Molecular Biology·Daniel N Wilson, Knud H Nierhaus
Aug 23, 2006·Journal of Bacteriology·Patricia SkorskiSylvie Hermann-Le Denmat
Dec 26, 2006·Nucleic Acids Research·Ryan K ShultzabergerThomas D Schneider
Jan 3, 2007·Journal of Molecular Biology·Alexey P KorepanovMikhail G Bubunenko
Apr 3, 2007·Journal of Bacteriology·India G Hook-BarnardMark A McIntosh
Nov 2, 2007·BMC Molecular Biology·Vladimir VimbergTanel Tenson
Nov 28, 2007·Journal of Bacteriology·Tim DurfeeDing Jun Jin
Feb 27, 2008·Biochemistry. Biokhimii︠a︡·A V KorobeinikovaM B Garber
Jun 4, 2008·Nature Reviews. Microbiology·Shanil P HaugenRichard L Gourse
Feb 17, 2009·Biochemistry. Biokhimii︠a︡·G M GongadzeM B Garber
May 16, 2009·Science·Seth R GoldmanBryce E Nickels
Feb 20, 2010·BMC Molecular Biology·Jacques Le DeroutEliane Hajnsdorf
May 7, 2010·Nucleic Acids Research·Cameron SmithRolf Backofen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.