Regulation of the Saccharomyces cerevisiae Slt2 kinase pathway by the stress-inducible Sdp1 dual specificity phosphatase.

The Journal of Biological Chemistry
Ji-Sook Hahn, Dennis J Thiele

Abstract

The Slt2/Mpk1 mitogen-activated protein kinase (MAPK) cell integrity pathway is involved in maintenance of cell shape and integrity during vegetative growth and mating in Saccharomyces cerevisiae. Slt2 is activated by dual phosphorylation of a threonine and tyrosine residue in response to several environmental stresses that perturb cell integrity. Negative regulation of Slt2 is achieved via dephosphorylation by two protein-tyrosine phosphatases, Ptp2 and Ptp3, and a dual specificity phosphatase, Msg5. In this study, we provide genetic and biochemical evidence that the stress-inducible dual specificity phosphatase, Sdp1, negatively regulates Slt2 by direct dephosphorylation. Deletion of SDP1 exacerbated growth defects due to overexpression of Mkk1(p386), a constitutively active mutant of Slt2 MAPK kinase, whereas overexpression of Sdp1 suppressed lethality caused by Mkk1(p386) overexpression. The heat shock-induced phosphorylation level of Slt2 was elevated in an sdp1Delta strain compared with that of the wild type, and heat shock-activated phospho-Slt2 was dephosphorylated by recombinant Sdp1 in vitro. Under normal growth conditions, an Sdp1-GFP fusion protein was localized to both the nucleus and cytoplasm. However, the Sdp1-G...Continue Reading

References

Jun 1, 1994·Current Opinion in Cell Biology·C Koch, K Nasmyth
Feb 1, 1994·Current Opinion in Genetics & Development·C J Marshall
Apr 1, 1997·Current Opinion in Cell Biology·M J Robinson, M H Cobb
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·J VernaR Ballester
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·M C GustinK Davenport
May 18, 1999·Molecular and Cellular Biology·M RajavelD E Levin
Dec 5, 2000·Molecular Biology of the Cell·A P GaschP O Brown
Apr 3, 2001·Nature Cell Biology·J C HarrisonD J Lew

❮ Previous
Next ❯

Citations

Mar 17, 2011·Molecular Genetics and Genomics : MGG·Carlos Romá-MateoRafael Pulido
Apr 30, 2009·The Journal of Biological Chemistry·Jingming ZhouMiriam L Greenberg
Oct 28, 2011·Molecular Biology of the Cell·Venkata K KuraviFrancis C Luca
May 5, 2012·Journal of Biochemistry·Seong Eon Ryu
May 12, 2012·Nucleic Acids Research·Mathieu CatalaSherif Abou Elela
Mar 6, 2007·Eukaryotic Cell·Eric M Rubenstein, Martin C Schmidt
Nov 16, 2010·Eukaryotic Cell·Joaquín AriñoAsier González
Jun 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·David E Levin
Jan 5, 2007·BMC Genomics·Kathy B SheehanLinda E Hyman
Dec 4, 2012·International Journal of Molecular Sciences·Jong H KimBruce C Campbell
Feb 21, 2014·Molecular Biology of the Cell·Chunyan JinKatrina F Cooper
May 18, 2011·The Journal of Cell Biology·Kai MaoDaniel J Klionsky
Mar 26, 2009·Journal of Photochemistry and Photobiology. B, Biology·Michèle DardalhonDietrich Averbeck
Jul 3, 2009·Fungal Genetics and Biology : FG & B·Nicolas RispailAntonio Di Pietro
Jan 8, 2008·Biochimica Et Biophysica Acta·Enrique HerreroElisa Cabiscol
Sep 17, 2005·Molecular Microbiology·Humberto MartínMaría Molina
Nov 19, 2003·Molecular Microbiology·Anusha Munasinghe GunasekeraDyann F Wirth
Apr 26, 2012·Molecular Systems Biology·Carl-Fredrik TigerMarcus Krantz
Jun 16, 2007·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·David S Perlin
Apr 29, 2015·Chemical Research in Toxicology·Upendarrao GollaRaghuvir S Tomar
Jul 3, 2007·Biochimica Et Biophysica Acta·Raymond E Chen, Jeremy Thorner
Sep 21, 2013·Biochemical and Biophysical Research Communications·Miwha ChangCheol-Won Yun
Nov 26, 2009·FEMS Yeast Research·Bárbara Martínez-BonoJuan Carlos Igual
Jun 30, 2012·FEMS Yeast Research·Ikram ZaidiMoez Hanin

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.