PMID: 8594195Feb 23, 1996Paper

Regulation of the Serratia marcescens extracellular nuclease: positive control by a homolog of P2 Ogr encoded by a cryptic prophage

Journal of Molecular Biology
S JinM J Benedik

Abstract

The Serratia marcescens extracellular nuclease is a secreted protein that is subject to growth phase and SOS control. Regulatory mutants affecting nuclease expression have been isolated that define a new locus, nucC, essential for transcription of the nuclease gene nucA. The cloned nucC gene is able to activate efficient expression from the nucA promoter in Escherichia coli, where it normally is poorly expressed. NucC is very similar to the bacteriophage P2 Ogr protein, a transcriptional activator essential for P2 late gene expression. NucC is able to replace P2 Ogr to support the growth of P2 ogr- mutants in E. coli. Ogr is a poor activator of the nuclease promoter in E. coli, but the related delta gene product from satellite phage P4 is highly effective. The presence of genes encoding a lysozyme and a putative porin or holin in the nucC operon suggests that nucC may be part of a cryptic prophage genome. The putative holin-like membrane protein is required in E. coli for extracellular secretion of the S. marcescens nuclease.

Citations

Aug 26, 1998·FEMS Microbiology Letters·M J Benedik, U Strych
Jul 31, 2003·Molecular Microbiology·Sherwood Casjens
Oct 6, 2000·Annual Review of Microbiology·I N WangR Young
May 19, 2009·Journal of Bacteriology·Victor J McAlister, Gail E Christie
Apr 29, 1998·Journal of Bacteriology·L J GuynnM J Benedik
Apr 5, 2001·Trends in Microbiology·N R WaterfieldR H ffrench-Constant
Dec 22, 1998·Gene·B JulienR Calendar
Jul 1, 1996·Journal of Bacteriology·Y SuhM J Benedik
Oct 1, 1996·Journal of Bacteriology·B Julien, R Calendar
Oct 23, 1997·Journal of Bacteriology·M BerkmenU Bläsi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.