Regulation of the Stress-Activated Degradation of Mitochondrial Respiratory Complexes in Yeast

Frontiers in Microbiology
Alba Timón-GómezMarkus Proft

Abstract

Repair and removal of damaged mitochondria is a key process for eukaryotic cell homeostasis. Here we investigate in the yeast model how different protein complexes of the mitochondrial electron transport chain are subject to specific degradation upon high respiration load and organelle damage. We find that the turnover of subunits of the electron transport complex I equivalent and complex III is preferentially stimulated upon high respiration rates. Particular mitochondrial proteases, but not mitophagy, are involved in this activated degradation. Further mitochondrial damage by valinomycin treatment of yeast cells triggers the mitophagic removal of the same respiratory complexes. This selective protein degradation depends on the mitochondrial fusion and fission apparatus and the autophagy adaptor protein Atg11, but not on the mitochondrial mitophagy receptor Atg32. Loss of autophagosomal protein function leads to valinomycin sensitivity and an overproduction of reactive oxygen species upon mitochondrial damage. A specific event in this selective turnover of electron transport chain complexes seems to be the association of Atg11 with the mitochondrial network, which can be achieved by overexpression of the Atg11 protein even in ...Continue Reading

References

Apr 18, 2000·Electrophoresis·H Y HongJ K Choi
Oct 17, 2003·Nature·Won-Ki HuhErin K O'Shea
Oct 17, 2003·Nature·Sina GhaemmaghamiJonathan S Weissman
Apr 17, 2004·Science·Enza Maria ValenteNicholas W Wood
Jul 13, 2004·The Journal of Biological Chemistry·Ingrid KissováNadine Camougrand
Jun 27, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Anastasia AndrukhivKeith D Garlid
Jun 15, 2007·Critical Reviews in Biochemistry and Molecular Biology·Mirko Koppen, Thomas Langer
May 6, 2008·Nature·Hector SandovalJin Wang
Aug 9, 2008·The Journal of Biological Chemistry·Vitaly A SelivanovTatyana V Votyakova
Sep 12, 2008·Advances in Experimental Medicine and Biology·David Lloyd
Dec 9, 2008·The Biochemical Journal·Michael P Murphy
Jul 22, 2009·Developmental Cell·Tomotake KankiDaniel J Klionsky
Dec 17, 2009·EMBO Reports·Ivana NovakIvan Dikic
Feb 4, 2010·PLoS Biology·Derek P NarendraRichard J Youle
May 22, 2010·Molecular Microbiology·Tomotake Kanki, Daniel J Klionsky
May 18, 2011·The Journal of Cell Biology·Kai MaoDaniel J Klionsky
Jul 16, 2011·Molecular Biology of the Cell·Yoshimasa AokiDongchon Kang
Jul 27, 2011·Journal of Visualized Experiments : JoVE·Dalibor MijaljicaRodney J Devenish
Jan 10, 2012·Current Biology : CB·Vincent SoubannierHeidi M McBride
Apr 16, 2013·Antioxidants & Redox Signaling·Evelina MaranzanaMaria Luisa Genova
Jun 5, 2013·Genetics·Fulvio Reggiori, Daniel J Klionsky
Jul 3, 2013·Science·Esther Lapuente-BrunJosé Antonio Enríquez
Jul 31, 2013·EMBO Reports·Tomotake KankiDongchon Kang
Nov 20, 2013·Biochimica Et Biophysica Acta·Maria Luisa Genova, Giorgio Lenaz
Dec 26, 2013·Biochimica Et Biophysica Acta·Rebeca Acin-Perez, Jose A Enriquez
Feb 18, 2014·Developmental Cell·Stefan Böckler, Benedikt Westermann
May 20, 2014·Journal of Cell Science·Masamune AiharaTomotake Kanki
Aug 2, 2014·Journal of Neurochemistry·Samantha GiordanoJianhua Zhang
Aug 6, 2014·Neuroscience Letters·Taku AmoNobutaka Hattori
Aug 12, 2014·The EMBO Journal·Ayumu SugiuraHeidi M McBride

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Citations

Oct 30, 2020·Metabolites·Erika M PalmieriDaniel W McVicar
Jan 9, 2021·Biochimica Et Biophysica Acta. Bioenergetics·Karolina Szczepanowska, Aleksandra Trifunovic
Feb 6, 2021·Biochimica Et Biophysica Acta. General Subjects·Yang Liu, Koji Okamoto

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Methods Mentioned

BETA
two-hybrid
two hybrid
fluorescence microscopy
immunoprecipitation

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