Regulation of the Vibrio vulnificus vvpE expression by cyclic AMP-receptor protein and quorum-sensing regulator SmcR

Microbial Pathogenesis
Choon Mee Kim, Sung-Heui Shin

Abstract

In Vibrio vulnificus, cAMP-receptor protein (CRP) and the quorum-sensing regulator SmcR are simultaneously and cooperatively required for the metalloprotease vvpE gene expression, rather than sequentially in a regulatory cascade. However, this study shows a new temporal and functional sequence between the two factors in regulating vvpE expression. A crp mutation inhibited vvpE expression with growth impairment from early stage. In contrast, a smcR mutation inhibited vvpE expression only at the late stage with no effect on growth. A crp-smcR double mutation severely inhibited vvpE expression with growth impairment from early stage. The inhibited vvpE expression was restored only at the early stage by a crp single complementation, but not at all by a smcR complementation. These results indicate that CRP functions as an essential activator, whereas SmcR functions in the presence of CRP for full vvpE expression.

References

Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G DittaD R Helinski
May 19, 2000·Infection and Immunity·C P Shao, L I Hor
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Regine Hengge-Aronis
May 7, 2003·Current Opinion in Microbiology·Karina B Xavier, Bonnie L Bassler
Oct 9, 2003·Applied and Environmental Microbiology·A HülsmannJ D Oliver
Oct 27, 2004·FEMS Microbiology Letters·Tomoka KawaseSumio Shinoda
Feb 3, 2005·FEMS Microbiology Letters·Young Ran KimJoon Haeng Rhee
Mar 24, 2006·FEMS Microbiology Letters·Mi-Hwa ChoiSung-Heui Shin
Jul 13, 2006·FEMS Immunology and Medical Microbiology·Mi-Hwa ChoiSung-Heui Shin
Mar 25, 2008·Current Opinion in Microbiology·Josef Deutscher

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.