Regulation of the Wilms' tumor gene during spermatogenesis

Developmental Dynamics : an Official Publication of the American Association of Anatomists
K Del Rio-TsonisP A Tsonis

Abstract

Spermatogenesis is the process by which male germ cells develop and mature, a pathway that includes a transition from a mitotic to a meiotic cell cycle. Throughout this pathway, the germ cells are in close contact with their nurturing cells, the Sertoli cells. Sertoli-germ cell interactions are difficult to study in mammals due to the complex cellular organization of their seminiferous tubules. The urodele amphibian testis, however, provides a unique system to study the process of germ cell maturation; it is organized in a gradient-like cystic structure, in which synchronized germ cells can be found within the same cyst. The Wilms' tumor gene (WT1) has been shown to be an essential gene for the formation of the gonads in mice, and it has been implicated in a variety of differentiation processes. The WT1 gene is thus a good candidate for the study of the differentiation processes involved in the maturation of the male germ cells. By using a probe for the urodele WT1 homologue in in situ hybridization studies, as well as an antibody against the WT1 protein in immunohistochemistry studies, we determined that WT1 gene expression in Sertoli cells depends on the stage of maturation of the associated germ cell. Thus, WT1 mRNA was dete...Continue Reading

References

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Citations

Mar 7, 2009·Reproductive Biology and Endocrinology : RB&E·Stéphane FlamentAmand Chesnel
Nov 11, 2009·Molecular Endocrinology·Zhiying HuMiles F Wilkinson
Sep 26, 2000·Human Molecular Genetics·N A LittleR C Davies
Aug 3, 2004·Microscopy Research and Technique·Francisco José SáezFrancisco Hernández
Dec 22, 2009·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Wusheng XiaoZhijun Zhou
May 31, 2019·Cell Communication and Signaling : CCS·Jingjing WangKang Zou

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