Abstract
The yeast SPS19 gene encoding the peroxisomally targeted 2,4-dienoyl-CoA reductase shares its promoter region (291 bp) with the sporulation-specific gene SPS18. SPS19 is induced during sporulation in diploids but to a lesser extent than SPS18; under oleate induction conditions, SPS19, but not SPS18, is transcribed via an oleate response element (ORE) independently of ploidy or sporulation. The SPS19 ORE is the binding target of the Pip2p and Oaf1p transcription factors, and an SPS19-lacZ reporter gene, which is highly expressed in oleate-induced cells, is not activated in haploids devoid of either protein. We examined the expression of CYC1-lacZ reporter constructs carrying the SPS19 and CTA1 OREs in diploids propagated under sporulation conditions and have shown that OREs are not sufficient for heterologous expression during yeast development. In addition, diploids deleted at either PIP2 or OAF1 demonstrated abundant ascosporogenesis, indicating that these genes are not essential for sporulation. A deltapex6 strain lacking peroxisomal structures and one devoid of fatty acyl-CoA oxidase (deltapox1), the first step in fungal beta-oxidation, were both proficient for sporulation and, hence, beta-oxidation and the peroxisomal compa...Continue Reading
Citations
Sep 22, 2001·Electrophoresis·H SchäferH E Meyer
Dec 10, 1999·The Biochemical Journal·A GurvitzH Rottensteiner
Feb 9, 2010·The Journal of Biological Chemistry·Sooraj Ratnakumar, Elton T Young
Apr 17, 2003·FEMS Microbiology Reviews·J Kalervo HiltunenAner Gurvitz
Sep 5, 2006·Biochimica Et Biophysica Acta·Aner Gurvitz, Hanspeter Rottensteiner
Feb 15, 2001·Molecular Cell Biology Research Communications : MCBRC·A GurvitzB Hamilton
Jul 9, 2009·FEMS Yeast Research·Aner GurvitzIan W Dawes
Dec 10, 2016·Molecular Biology of the Cell·Tzu-Han HsuChao-Wen Wang
Sep 20, 2017·The Journal of Biological Chemistry·Praveen Kumar RajvanshiRam Rajasekharan
Feb 21, 2008·The Journal of Biological Chemistry·Igor V KarpichevGillian M Small
Aug 24, 1999·The Journal of Biological Chemistry·A GurvitzJ K Hiltunen
Jul 31, 1999·The Journal of Biological Chemistry·U BaumgartnerH Rottensteiner
Jun 30, 2001·The Journal of Biological Chemistry·A GurvitzH Rottensteiner
Nov 13, 1998·The Journal of Biological Chemistry·A GurvitzH Rottensteiner
Oct 18, 2000·The Journal of Biological Chemistry·A GurvitzA Hartig
May 16, 2003·The Journal of Biological Chemistry·Hanspeter RottensteinerAner Gurvitz
Sep 20, 2006·The Journal of Biological Chemistry·Simon GoepfertYves Poirier
Nov 26, 2010·Applied and Environmental Microbiology·Syed A HasanDick B Janssen
Jun 9, 2009·IUBMB Life·Hiderou Yoshida