PMID: 6105961Aug 1, 1980Paper

Regulation of thiolases from pig heart. Control of fatty acid oxidation in heart

European Journal of Biochemistry
Y Olowe, H Schulz

Abstract

The effects of various mitochondrial coenzymes and metabolities on the activities of 3-oxoacyl-CoA thiolase (EC 2.3.1.16) and acetoacetyl-CoA thiolase (EC 2.3.1.9) from pig heart were investigated with the aim of elucidating the possible regulation of these two enzymes. Of the compounds tested, acetyl-CoA was the most effective inhibitor of both thiolases. However, 3-oxoacyl-CoA thiolase was more severly inhibited by acetyl-CoA than was acetoacetyl-CoA thiolase. 3-Oxoacyl-CoA thiolase was also significantly inhibited by decanoyl-CoA while acetoacetyl-CoA thiolase was inhibited by 3-hydroxybutyryl-CoA as strongly as it was by acetyl-CoA. All other compounds either did not affect the thiolase activities or only at unphysiologically high concentrations. The inhibition of acetoacetyl-CoA thiolase by acetyl-CoA was linear and apparently noncompetitive with respect to CoASH (Ki = 125 microM) whereas that of 3-oxoacyl-CoA thiolase was nonlinear. However at low concentrations of acetyl-CoA the inhibition of 3-oxoacyl-CoA thiolase was linear competitive with respect to CoASH (Ki = 3.9 microM). It is concluded that 3-oxoacyl-CoA thiolase, but not acetoacetyl-CoA thiolase, will be completely inhibited by acetyl-CoA at concentrations of Co...Continue Reading

References

Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J F BinstockH Schulz
Jul 1, 1975·The Biochemical Journal·K K Stanley, P K Tubbs
Sep 1, 1953·Biochimica Et Biophysica Acta·F LYNEN, S OCHOA
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Jan 1, 1974·Annual Review of Physiology·J R Neely, H E Morgan

❮ Previous
Next ❯

Citations

Apr 24, 2008·American Journal of Physiology. Endocrinology and Metabolism·Nam Ho Jeoung, Robert A Harris
Jun 1, 1985·Metabolism: Clinical and Experimental·J T Brosnan, K Reid
Jun 6, 2012·Oxidative Medicine and Cellular Longevity·Abdelkader E AshourAbdulqader Alhaider
Sep 11, 1991·Archives of Biochemistry and Biophysics·H Y WangH Schulz
May 15, 1988·Analytical Biochemistry·P M LatipääJ K Hiltunen
Mar 1, 1990·The American Journal of Physiology·C Des RosiersH Brunengraber
Jan 30, 2002·Progress in Lipid Research·Simon Eaton
Aug 4, 1994·Biochimica Et Biophysica Acta·G D LopaschukB O Schönekess
Apr 23, 1993·Biochimica Et Biophysica Acta·M Guzmán, M J Geelen
Jan 28, 1991·Biochimica Et Biophysica Acta·H Schulz
Jan 1, 1982·Archives of Biochemistry and Biophysics·B Davidson, H Schulz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.