Regulation of thyroperoxidase, thyroglobulin and iodide levels in sheep thyroid cells by TSH, tumor promoters and epidermal growth factor

Biochimie
M A PrattG N Burrow

Abstract

Using sheep thyroid cells in culture, we have studied the effects of thyroid stimulating hormone (TSH), epidermal growth factor (EGF) and the tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA) on the activity and expression of both thyroglobulin (Tg) and thyroid peroxidase (TPO) and on the ability of cells to trap and organify iodide. Using Western blotting techniques, we found that TSH increased the absolute cellular levels of Tg. The optimum TSH concentration for Tg mRNA production was between 0.1-1.0 mU/ml. Thyroglobulin mRNA levels were stimulated by TSH but detectable levels were also present in cultures grown in its absence containing cortisol, insulin, transferrin, somatostatin and glycyl-lysyl-histidyl acetate. Unlike Tg, TPO protein levels were found to be completely dependent upon TSH. A time course of TSH stimulation of TPO mRNA showed increases after 8 h of TSH stimulation, whereas induction of Tg mRNA by TSH was seen at 24 h. Iodide trapping and organification were also TSH-dependent processes, showing maximum activities at 300-500 muU/ml of TSH. The addition of 10 nM TPA caused a biphasic decrease in radiolabeled pertechnetate uptake, with complete inhibition being seen at 14 h. Inhibition of iodide organi...Continue Reading

Citations

Aug 1, 1994·Journal of Cellular Physiology·S DesruisseauO Chabaud
Jul 1, 1995·Trends in Endocrinology and Metabolism : TEM·M Nilsson
Apr 6, 2001·Thyroid : Official Journal of the American Thyroid Association·U MartiU Bürgi
Dec 1, 1991·Baillière's Clinical Endocrinology and Metabolism·J E DumontP P Roger
Jun 20, 2015·International Journal of Endocrinology·Ana Paula Santin BertoniTania Weber Furlanetto
Jan 1, 1992·Progress in Histochemistry and Cytochemistry·E Raspé, J E Dumont

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