Regulation of TLR4 expression mediates the attenuating effect of erythropoietin on inflammation and myocardial fibrosis in rat heart

International Journal of Molecular Medicine
Fei LiuJingtian Peng

Abstract

The mechanism underlying the anti‑inflammatory or antifibrotic activity of erythropoietin (EPO) in myocardial fibrosis (MF) remains elusive. In the current study, abdominal aortic constriction (AAC) was performed on rats and EPO and/or Toll‑like receptor (TLR)4 were overexpressed in rat hearts through intramyocardial administration of lentivirus expressing the EPO and TLR4 genes. Hematoxylin and eosin staining and Masson's trichrome staining were performed on tissue sections from rat hearts for histopathological examination. ELISA was used to determine the levels of inflammatory mediators in serum. Gene expression levels were determined by quantitative polymerase chain reaction analysis and protein expression levels were determined by western blot analysis and immunofluorescence staining. The results indicated that EPO overexpression improved MF in rat hearts, by inhibiting the release of transforming growth factor (TGF)‑β1, tumor necrosis factor (TNF)‑α, interleukin (IL)‑6, IL‑1β, IL‑17A, matrix metalloproteinase (MMP)‑9 and MMP‑2. Moreover, EPO overexpression suppressed the expression of TLR4, while promoting phosphoinositide 3‑kinase (PI3K) and phosphorylated AKT serine/threonine kinase 1 (Akt) expression levels. However, th...Continue Reading

References

Sep 20, 1984·Nature·R StewardP Schedl
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 3, 2010·Journal of the American Society of Nephrology : JASN·Wilco P PulskensJaklien C Leemans
Nov 18, 2010·Advances in Pharmacology·Anantha Vijay R SanthanamZvonimir S Katusic
Apr 9, 2011·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Matilde LombarderoBernd W Scheithauer
Jun 27, 2014·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Su-Feng ZhouXiang Cheng
Dec 3, 2014·European Journal of Pharmacology·Ji Hae JunYoung-Lan Kwak
Jan 13, 2015·Molecular Medicine Reports·Xu Chang GengGuo Yong Lian
Apr 12, 2015·Scandinavian Journal of Gastroenterology·Shinji NakamuraYoshiyuki Nakajima
Jul 15, 2015·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Thomas Moore-MorrisSylvia M Evans
Oct 23, 2015·International Journal of Molecular Sciences·Jun WangYushun Zhang
Oct 29, 2015·BMC Complementary and Alternative Medicine·Xiang-Lan WeiWei-Jin Zang
Mar 19, 2016·Circulation Research·Joshua G TraversBurns C Blaxall
May 27, 2016·Cell Death & Disease·Y YangW Yi
Jun 9, 2016·Experimental Lung Research·Ali Haydar TurhanMehmet Ali Sungur
Jun 11, 2016·Experimental and Therapeutic Medicine·Weibin QiSumin Wang

❮ Previous
Next ❯

Citations

Dec 17, 2020·Frontiers in Cardiovascular Medicine·Zheng XiaoHe Huang
Jul 25, 2021·International Journal of Molecular Sciences·Zuzana TóthováPeter Solár
Aug 10, 2021·Frontiers in Pharmacology·Wenkai YangJianguo Wu

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
density gradient centrifugation
PCR

Software Mentioned

Quantity One
7500
Premier Primer
Image Pro Plus
SPSS

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.