PMID: 3756161Oct 9, 1986Paper

Regulation of transbilayer distribution of a fluorescent sterol in tumor cell plasma membranes

Biochimica Et Biophysica Acta
A B KierF Schroeder

Abstract

The lipid composition and transbilayer distribution of plasma membrane isolated from primary tumor (L-929, LM, A-9 and C3H) and nine metastatic cell lines cultured under identical conditions was examined. Cultured primary tumor and metastatic cells differed two-fold in sterol/phospholipid molar ratios. There was a direct correlation between plasma membrane anionic phospholipid (phosphatidylinositol and phosphatidylserine) content and plasma membrane sterol/phospholipid ratio. This finding may bear on the possible link between oncogenes and inositol lipids. The fluorescent sterol, dehydroergosterol, was incorporated into primary tumor and metastatic cell lines. Selective quenching of outer monolayer fluorescence by covalently linked trinitrophenyl groups demonstrated an asymmetric transbilayer distribution of sterol in the plasma membranes. The inner monolayer of the plasma membranes from both cultured primary and metastatic tumor cells was enriched in sterol as compared with the outer monolayer. Consistent with this, the inner monolayer was distinctly more rigid as determined by the limiting anisotropy of 1,6-diphenyl-1,3,5-hexatriene. Dehydroergosterol fluorescence was temperature dependent and sensitive to lateral phase separ...Continue Reading

References

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Citations

Jul 31, 2004·Archives of Pharmacal Research·Moon-Kyoung BaeIl Yun
Sep 1, 1988·Biochimica Et Biophysica Acta·G NemeczF Schroeder
Dec 22, 1988·Biochimica Et Biophysica Acta·G Smutzer
Dec 8, 1988·Biochimica Et Biophysica Acta·F SchroederW G Wood
Feb 19, 1988·Biochimica Et Biophysica Acta·G Smutzer
Aug 4, 1994·Biochimica Et Biophysica Acta·P J Trotter, D R Voelker
Nov 1, 1990·Chemistry and Physics of Lipids·I HapalaF Schroeder
Oct 1, 1988·Biophysical Chemistry·F SchroederT E Thompson
Aug 19, 2011·American Journal of Physiology. Endocrinology and Metabolism·Stephen M StoreyBarbara P Atshaves
Aug 24, 2012·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Hyung-Jin JooIl Yun

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