PMID: 11933077Apr 5, 2002Paper

Regulation of transcription of the intracellular interleukin-1 receptor antagonist gene by AP-1 in mouse carcinoma cells

Molecular Carcinogenesis
Eunhye LaSusan M Fischer

Abstract

Interleukin-1 receptor antagonist (IL-1Ra) is involved in many processes, including epidermal inflammation and hyperplasia after irritation or injury. However, the mechanism by which intracellular IL-1Ra (icIL-1Ra) expression is regulated in mouse keratinocytes has not been reported. We found that the CH72 mouse carcinoma cell line constitutively expresses the icIL-1Ra mRNA. To study the transcriptional factors responsible for the constitutive expression of icIL-1Ra, we functionally characterized 4.5 kb of the 5' flanking region of the human icIL-1Ra gene in these cells. We first demonstrated that icIL-1Ra expression in these cells was regulated at the level of transcription. Deletion analysis of the promoter showed that regulatory elements for constitutive expression were located -158 to -49 bp upstream of the transcription start site for icIL-1Ra. We investigated the cis- and trans-acting factors required for icIL-1Ra expression. An activating protein-1 (AP-1) site was identified as the positive regulatory element necessary for the constitutive expression of the icIL-1Ra promoter in CH72 cells. Moreover, electrophoretic mobility shift assay and cotransfection experiments showed that c-jun and c-fos proteins bound to the AP-1 ...Continue Reading

References

May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S HaskillS Fong
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S P EisenbergR C Thompson
Nov 1, 1991·The Journal of Clinical Investigation·W P Arend
Sep 1, 1990·Metabolism: Clinical and Experimental·J F HabenerJ P Hoeffler
Sep 17, 1996·International Journal of Cancer. Journal International Du Cancer·R G ChiriviR Giavazzi
Apr 1, 1997·Current Opinion in Cell Biology·M KarinE Zandi
May 23, 1998·Annual Review of Immunology·W P ArendC Gabay
May 13, 1999·The Journal of Biological Chemistry·D GuptaR Dziarski
Mar 4, 2000·Progress in Nucleic Acid Research and Molecular Biology·D De Cesare, P Sassone-Corsi

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Citations

Aug 17, 2005·Journal of Virology·Milena Iacobelli-MartinezGlen R Nemerow
Jun 16, 2005·The Journal of Investigative Dermatology·John B MeeRichard W Groves
Jun 20, 2003·Journal of Biochemical and Molecular Toxicology·James V RogersJames N McDougal
Jun 26, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tiehui WangChristopher J Secombes

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