PMID: 7540092Mar 1, 1995Paper

Regulation of transepithelial ion transport by two different purinoceptors in the apical membrane of canine kidney (MDCK) cells

British Journal of Pharmacology
O Zegarra-MoranL J Galietta

Abstract

1. The effect of extracellular nucleotides on the transepithelial ion transport of Madin Darby canine kidney cells (MDCK) was investigated. Cells were grown up to confluency on permeable supports and the short circuit current (ISC) was measured with an Ussing chamber-like mini-perfusion system. 2. Apical ATP stimulated a biphasic ISC increase consisting of a first rapid and transient peak followed by a broader one. 3. The first peak evoked by ATP was reversibly blocked by basilen blue (BB) in a concentration-dependent fashion, with an EC50 of 7.5 microM. 4. The P2 gamma receptor agonist, 2-methylthioATP (2-MeSATP) caused a single transient ISC increase that was completely blocked by pretreatment with BB. On the contrary, the P2x agonist, alpha, beta-methylene ATP (alpha, beta-meATP) was almost completely ineffective on ISC. UTP essentially induced a monophasic response the time-course of which resembled that of the second peak stimulated by ATP. The agonist potency order was 2-MeSATP > or = ATP > UTP, alpha, beta-meATP for the first peak and UTP > or = ATP > 2-MeSATP > alpha, beta-meATP for the second peak. 5. Monolayer incubation with the membrane permeable calcium chelator [bis-o-aminophenoxy)-ethane-N,N,N',N',-tetraacetic ac...Continue Reading

References

Jan 1, 1992·The American Journal of Physiology·S DhoJ K Foskett
Apr 1, 1991·Trends in Pharmacological Sciences·S E O'ConnorP Leff
May 1, 1982·The Journal of Cell Biology·D A HerzlingerG K Ojakian
Aug 20, 1981·Biochimica Et Biophysica Acta·N L Simmons
Jan 1, 1994·Trends in Pharmacological Sciences
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K D LustigD Julius
Apr 1, 1994·Pflügers Archiv : European journal of physiology·L J GaliettaG Romeo

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Citations

Apr 1, 1997·Cell Biology International·W Zhang, G M Roomans
Nov 21, 2007·Pflügers Archiv : European journal of physiology·C S GeytiH A Praetorius
Jun 10, 2009·Purinergic Signalling·Clare M TurnerFrederick W K Tam
Apr 25, 2012·Purinergic Signalling·Jenny Öhman, David Erlinge
Nov 19, 1997·Neuropharmacology·J B SchachterT K Harden
May 8, 2001·Clinical and Experimental Pharmacology & Physiology·P A InselS R Post
Nov 23, 2013·Purinergic Signalling·Geoffrey BurnstockMatthew A Bailey
Aug 24, 1999·The Journal of Physiology·U BanderaliJ Ehrenfeld
Mar 3, 2007·Biochemical Pharmacology·Keisuke MigitaTakeshi Katsuragi
Apr 12, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J T TurnerB R Talamo
Dec 15, 2000·Acta Physiologica Scandinavica·T Hafting, O Sand
Jun 8, 2007·Journal of the American Society of Nephrology : JASN·Mikkel Erik Juul JensenJens Leipziger
Jul 20, 2006·American Journal of Physiology. Renal Physiology·Clare M TurnerRobert J Unwin
Oct 27, 1999·Canadian Journal of Physiology and Pharmacology·F GagnonS N Orlov
Nov 30, 2000·American Journal of Physiology. Renal Physiology·A C ZambonP A Insel
Aug 5, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·C M YangC C Wang
Jan 31, 2003·American Journal of Physiology. Renal Physiology·Jens Leipziger
May 15, 2001·American Journal of Physiology. Cell Physiology·J E Smitham, K E Barrett
Aug 9, 2002·American Journal of Physiology. Renal Physiology·Craig B WodaLisa M Satlin
Jul 15, 2000·American Journal of Physiology. Renal Physiology·I RuberaP Poujeol
Dec 22, 1999·The American Journal of Physiology·L S NadlerN M Nathanson
Sep 16, 2004·American Journal of Physiology. Renal Physiology·H A PraetoriusJ Leipziger
Apr 13, 1999·The American Journal of Physiology·L L ClarkeG A Weisman

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