PMID: 16629180Apr 25, 2006Paper

Regulation of transepithelial transport of iron by hepcidin

Biological Research
Natalia P MenaMarco T Núñez

Abstract

Hepcidin (Hepc) is a 25 amino acid cationic peptide with broad antibacterial and antifungal actions. A likely role for Hepc in iron metabolism was suggested by the observation that mice having disruption of the gene encoding the transcription factor USF2 failed to produce Hepc mRNA and developed spontaneous visceral iron overload. Lately, Hepc has been considered the "stores regulator," a putative factor that signals the iron content of the body to intestinal cells. In this work, we characterized the effect of Hepc produced by hepatoma cells on iron absorption by intestinal cells. To that end, human Hepc cDNA was cloned and overexpressed in HepG2 cells and conditioned media from Hepc-overexpressing cells was used to study the effects of Hepe on intestinal Caco-2 cells grown in bicameral inserts. The results indicate that Hepc released by HepG2 inhibited apical iron uptake by Caco-2 cells, probably by inhibiting the expression of the apical transporter DMT1. These results support a model in which Hepc released by the liver negatively regulates the expression of transporter DMT1 in the enterocyte.

Citations

Nov 19, 2009·Neurotoxicity Research·Vanessa A FitsanakisMichael Aschner
Feb 9, 2016·Biochemical and Biophysical Research Communications·Xiaoqin LuoNanping Wang
Aug 18, 2009·The American Journal of Emergency Medicine·Michal ToledanoMatitiahu Berkovitch
Dec 30, 2006·Trends in Cell Biology·Louise L DunnDes R Richardson
Sep 10, 2011·Hematology·Sahika Zeynep AkıGülsan Türköz Sucak
Dec 21, 2007·Nature Clinical Practice. Nephrology·Karien van der PuttenCarlo A J M Gaillard
Dec 20, 2011·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Pantelis A SarafidisIain C Macdougall

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