PMID: 6104988Jul 15, 1980Paper

Regulation of transglutaminase activity in Chinese hamster ovary cells

Biochimica Et Biophysica Acta
P G MilhaudM M Gottesman

Abstract

We have investigated the regulation of transglutaminase activity (epsilon-(gamma-glutamyl)lysine crosslinking enzyme) in Chinese hamster ovary cells in culture. We report that transglutaminase activity increases several-fold in CHO cells at maximum density in suspension culture. This increase cannot be explained by the presence of soluble regulators of the enzyme activity or the appearance of a new enzyme activity with a different affinity for substrate, but appears to be due to an increase in total enzyme activity. Treatment of CHO cells at low cell density with 8-bromo cyclic AMP results in a small increase (20--70%) in transglutaminase activity. By studying CHO mutants which have altered or absent cyclic-AMP-dependent protein kinases, we have demonstrated that the effect of cyclic AMP on transglutaminase activity at low cell density is mediated by cyclic-AMP-dependent protein kinase. However, the protein kinase mutants show normal increases in transglutaminase activity at high cell density, indicating that cyclic AMP-dependent protein kinase does not mediate density-dependent changes in transglutaminase activity.

References

Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A NovogrodskyK H Stenzel
Nov 1, 1978·Archives of Biochemistry and Biophysics·M F PlishkerL A Goldsmith
Jan 1, 1977·Advances in Protein Chemistry·J E Folk, J S Finlayson
Jun 21, 1979·Nature·J L GoldsteinM S Brown
Jan 1, 1977·Journal of Supramolecular Structure·E F NeufeldL H Rome
Dec 1, 1972·Analytical Biochemistry·L LorandL Cooperstein
Jan 1, 1980·Somatic Cell Genetics·M M GottesmanI Pastan

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Citations

Jul 15, 1991·Experientia·S Kawashima
Sep 1, 1985·Journal of Cellular Physiology·G Korner, U Bachrach
Jan 1, 1986·Annals of the New York Academy of Sciences·M M GottesmanI Abraham
Dec 14, 1995·Biochimica Et Biophysica Acta·A SessaA Perin
Sep 1, 1987·Molecular and Cellular Biology·I AbrahamJ K Mayo

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