Regulation of transmitter release by synapsin II in mouse motor terminals

The Journal of Physiology
Dmitry SamigullinM Bykhovskaia

Abstract

We investigated quantal release and ultrastructure in the neuromuscular junctions of synapsin II knockout (Syn II KO) mice. Synaptic responses were recorded focally from the diaphragm synapses during electrical stimulation of the phrenic nerve. We found that synapsin II affects transmitter release in a Ca(2+)-dependent manner. At reduced extracellular Ca(2+) (0.5 mM), Syn II KO mice demonstrated a significant increase in evoked and spontaneous quantal release, while at the physiological Ca(2+) concentration (2 mM), quantal release in Syn II KO synapses was unaffected. Protein kinase inhibitor H7 (100 microM) suppressed quantal release significantly stronger in Syn II KO synapses than in wild type (WT), indicating that Syn II KO synapses may compensate for the lack of synapsin II via a phosphorylation-dependent pathway. Electron microscopy analysis demonstrated that the lack of synapsin II results in an approximately 40% decrease in the density of synaptic vesicles in the reserve pool, while the number of vesicles docked to the presynaptic membrane remained unchanged. Synaptic depression in Syn II KO synapses was slightly increased, which is consistent with the depleted vesicle store in these synapses. At reduced Ca(2+) frequenc...Continue Reading

References

Sep 1, 1992·The Journal of Physiology·A I Bain, D M Quastel
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R LlinásP Greengard
Feb 1, 1966·The Journal of Cell Biology·G F Gauthier, H A Padykula
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·A FerreiraK S Kosik
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·L LiP Greengard
Jun 8, 1995·Nature·V A PieriboneP Greengard
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y Goda, C F Stevens
Apr 1, 1996·Journal of Neuroscience Methods·M BykhovskaiaJ T Hackett
Jan 27, 1998·The Journal of Biological Chemistry·M Hosaka, T C Südhof
Nov 10, 1998·Neuron·C F Stevens, J M Sullivan
Apr 9, 1999·Nature Neuroscience·S HilfikerG J Augustine
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·S HilfikerP Greengard
Mar 22, 2000·Nature Neuroscience·J N JovanovicT S Sihra
May 8, 2000·Journal of Computational Neuroscience·M BykhovskaiaJ T Hackett
Jan 9, 2001·Brain Research. Molecular Brain Research·T SugiyamaT Yoshioka
Nov 28, 2001·Nature Neuroscience·V N Murthy
Feb 5, 2002·Annual Review of Physiology·Robert S Zucker, Wade G Regehr
May 20, 2003·The Journal of Biological Chemistry·Michael A CousinPhillip J Robinson
May 21, 2003·The Journal of Cell Biology·Ona BloomOleg Shupliakov
Mar 27, 2004·Science·Silvio O Rizzoli, William J Betz
Jul 9, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tei-ichi Nishiki, George J Augustine
Aug 13, 2004·Brain Research·Maria BykhovskaiaJohn T Hackett

❮ Previous
Next ❯

Citations

Jul 20, 2010·Journal of Molecular Neuroscience : MN·Gyula AcsadiGraham C Parker
Oct 24, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Daniel GitlerGeorge J Augustine
Jan 23, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lingling KongCharlotte J Sumner
Dec 26, 2009·Cellular and Molecular Life Sciences : CMLS·Eugenio F FornasieroFlavia Valtorta
Aug 11, 2011·Seminars in Cell & Developmental Biology·Maria Bykhovskaia
Mar 27, 2010·Molecular and Cellular Neurosciences·William L Coleman, Maria Bykhovskaia
Nov 30, 2007·Journal of Neuroscience Methods·Maria Bykhovskaia
Aug 11, 2015·The Journal of Physiology·Maxim Nikolaev, Paul Heggelund
Nov 10, 2011·Journal of Neurochemistry·Ayelet OrenbuchGitler Daniel
May 26, 2017·Wiley Interdisciplinary Reviews. Developmental Biology·Maria Bykhovskaia, Alexander Vasin
Aug 19, 2021·Neuroscience and Biobehavioral Reviews·Francesca LonghenaArianna Bellucci

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved