PMID: 9531479Jun 11, 1998Paper

Regulation of type I collagen mRNA in lung fibroblasts by cystine availability

The Biochemical Journal
D C RishikofRonald H Goldstein

Abstract

The steady-state level of alpha1(I) collagen mRNA is regulated by amino acid availability in human lung fibroblasts. Depletion of amino acids decreases alpha1(I) collagen mRNA levels and repletion of amino acids induces rapid re-expression of alpha1(I) mRNA. In these studies, we examined the requirements for individual amino acids on the regulation of alpha1(I) collagen mRNA. We found that re-expression of alpha1(I) collagen mRNA was critically dependent on cystine but not on other amino acids. However, the addition of cystine alone did not result in re-expression of alpha1(I) collagen mRNA. Following amino acid depletion, the addition of cystine with selective amino acids increased alpha1(I) collagen mRNA levels. The combination of glutamine and cystine increased alpha1(I) collagen mRNA levels 6.3-fold. Methionine or a branch-chain amino acid (leucine, isoleucine or valine) also acted in combination with cystine to increase alpha1(I) collagen mRNA expression, whereas other amino acids were not effective. The prolonged absence of cystine lowered steady-state levels of alpha1(I) collagen mRNA through a mechanism involving decreases in both the rate of gene transcription as assessed by nuclear run-on experiments and mRNA stabilit...Continue Reading

Citations

Jul 14, 2010·Progress in Histochemistry and Cytochemistry·Philippe G CammisottoMoise Bendayan
Jun 23, 2016·Metabolomics : Official Journal of the Metabolomic Society·Polona ŽigonSnezna Sodin-Semrl
Feb 23, 2007·Critical Care : the Official Journal of the Critical Care Forum·Maria-Eudóxia Pilotto de CarvalhoDaniel Deheinzelin
Oct 24, 1998·Biochimica Et Biophysica Acta·D C RishikofR H Goldstein
Aug 21, 2010·The American Journal of Pathology·Mariya T SweetwyneJoanne E Murphy-Ullrich
Jul 13, 2011·Molecular and Cellular Biology·Azariyas A Challa, Branko Stefanovic
Sep 24, 2005·American Journal of Respiratory and Critical Care Medicine·Peter R A JohnsonJudith L Black
Dec 7, 2002·American Journal of Respiratory and Critical Care Medicine·Annie PardoMoisés Selman
Mar 7, 2017·Biochemistry. Biokhimii︠a︡·N SandboN O Dulin
Sep 28, 2000·Journal of Applied Physiology·J L BerkR H Goldstein

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