Regulation of type IV secretion apparatus genes during Ehrlichia chaffeensis intracellular development by a previously unidentified protein

Journal of Bacteriology
Zhihui ChengYasuko Rikihisa

Abstract

The type IV secretion (T4S) system is critical for the virulence of several pathogens. In the rickettsial pathogen Ehrlichia chaffeensis, the virBD genes are split into two operons, the virB3-virB6 (preceded by sodB) and virB8-virD4 operons. Between these two operons, there are duplications of virB4, virB8, and virB9. In this study we found that transcription of all five loci was downregulated prior to the release of E. chaffeensis from host THP-1 cells and was upregulated at the initiation of exponential growth. Electrophoretic mobility shift assays revealed an E. chaffeensis-encoded protein that specifically bound to the promoter regions upstream of the virBD loci. The protein was purified from the bacterial lysate by affinity chromatography using a biotinylated promoter region upstream of sodB. Mass spectrometry identified the protein as an E. chaffeensis 12.3-kDa hypothetical protein, which was designated EcxR. Recombinant EcxR bound to the promoter regions upstream of five individual virBD loci. EcxR also activated transcription of all five virBD loci in lacZ reporter constructs. The expression of ecxR was positively autoregulated by EcxR. These results suggest that the five virBD loci are coordinately regulated by EcxR to...Continue Reading

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Citations

Oct 22, 2013·Microbes and Infection·Paige Selvy DunphyJere W McBride
Apr 28, 2012·The Journal of Infectious Diseases·Mohd Akhlakur RahmanYasuko Rikihisa
Feb 24, 2010·Infection and Immunity·Joseph J GillespieBruno W Sobral
Feb 9, 2010·Journal of Bacteriology·Wilmara Salgado-PabónJoseph P Dillard
Dec 1, 2009·Microbiology and Molecular Biology Reviews : MMBR·Cristina E Alvarez-Martinez, Peter J Christie
Aug 30, 2014·FEMS Microbiology Reviews·Joseph J GillespieAbdu F Azad
Dec 1, 2015·Microbes and Infection·Amal Moumène, Damien F Meyer
Jun 11, 2009·Molecular Microbiology·Matxalen LlosaChristoph Dehio
Jun 23, 2009·Annals of the New York Academy of Sciences·Yasuko RikihisaZhihui Cheng
Jul 31, 2010·Cellular Microbiology·Yasuko RikihisaHua Niu
Aug 27, 2014·Molecular Microbiology·Gerald D BaldridgeAnn M Fallon
Jun 16, 2016·Frontiers in Cellular and Infection Microbiology·Taslima T LinaJere W McBride
Oct 22, 2015·Annual Review of Microbiology·Yasuko Rikihisa
Apr 11, 2018·Frontiers in Microbiology·Ruiping ZhengZhihui Cheng
Jun 3, 2021·Proceedings of the National Academy of Sciences of the United States of America·Qi YanYasuko Rikihisa

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