PMID: 7542754Jan 1, 1995Paper

Regulation of U-937 monocyte adhesion to cultured human mesangial cells by cytokines and vasoactive agents

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
P MenèK H Thierauch

Abstract

Leukocyte adhesion to kidney cells is an early event in renal inflammation, such as glomerulonephritis. We developed an experimental model of monocyte adhesion to cultured human mesangial cells. U-937 myelomonocytic leukaemia cells, similar to peripheral blood human monocytes, irreversibly bound to mesangial cell monolayers upon 30-180 min coincubations (to a max. of 13,600 +/- 1100/cm2 monolayer), as assessed by cell counting, U-937 labelling with 3H-thymidine, and colorimetry of nuclear staining with crystal violet. Adhesion was enhanced in mesangial cells proliferating in response to 17% fetal bovine serum, indicating expression of a proinflammatory phenotype. E. coli lipopolysaccharide (LPS), tumour necrosis factor-alpha (TNF-alpha) and protein kinase C activation with phorbol myristate acetate (PMA) potentiated monocyte binding during either coincubation or 24-h pretreatment (0.1 microM PMA, +200 +/- 21%). Binding was also promoted by pretreatment with vasoconstrictors, such as the thromboxane A2 mimetic, U-46619 (10 nM-1 microM, max. +35 +/- 3%), or 1 microM angiotensin II (+64 +/- 4%). To elucidate the mechanisms of monocyte adhesion, we analysed the adhesion molecules expressed by human mesangial cells, employing revers...Continue Reading

Citations

May 1, 2001·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·P MenèG Gambaro
Jan 12, 2011·Investigational New Drugs·Claudriana LocatelliTânia Beatriz Creczynski-Pasa
Aug 26, 1999·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·T NagasakiS Otani
May 9, 2001·Current Opinion in Nephrology and Hypertension·M Ruiz-OrtegaJ Egido
Dec 18, 2003·The Journal of Biological Chemistry·Aimin Wang, Vincent C Hascall
Jan 5, 2002·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Paolo MenèGiulio A Cinotti
Apr 7, 1997·Biochemical and Biophysical Research Communications·T NagasakiS Otani
Mar 13, 2003·Kidney International·Ravinder S ChanaDavid C Wheeler
Apr 5, 2003·The International Journal of Biochemistry & Cell Biology·Yusuke SuzukiJesus Egido
Aug 1, 1997·Kidney International·S HeidenreichM R Daha

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