PMID: 2124141Dec 6, 1990Paper

Regulation of urea synthesis in sublobular regions of the liver lobule by oxygen

Biochimica Et Biophysica Acta
Y TakeiR G Thurman

Abstract

Periportal and pericentral regions of the liver lobule were isolated from perfused rat liver using a micropunch and incubated in Krebs-Henseleit buffer (pH 7.6) containing 2% poly(ethylene glycol) in Eagle's basal medium, PMSF (50 micrograms/ml) and leupeptin (20 micrograms/ml) for 2 h at 25 degrees C under and O2/CO2 (95:5%) gas phase. Maximal rates of urea production from ammonium chloride were 96.4 +/- 8.7 and 32.8 +/- 5.4 mumol/g per h at 800 and 200 microM O2. Thus, urea synthesis was 2-3-times greater at high than low O2 tension in plugs from periportal and pericentral regions of the liver lobule.

References

Aug 30, 1988·Biochemical and Biophysical Research Communications·U K MisraR G Thurman
Jan 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D Haüssinger
Mar 1, 1988·Journal of Pediatric Orthopedics·P M Stevens
Jan 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R G Thurman, F C Kauffman
Sep 15, 1973·The Journal of Comparative Neurology·D Pfaff, M Keiner

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Citations

Oct 1, 1992·Alcoholism, Clinical and Experimental Research·U K MisraR G Thurman

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