Regulation of UT-A1-mediated transepithelial urea flux in MDCK cells.

American Journal of Physiology. Cell Physiology
Otto FröhlichRobert B Gunn

Abstract

Transepithelial [(14)C]urea fluxes were measured across cultured Madin-Darby canine kidney (MDCK) cells permanently transfected to express the urea transport protein UT-A1. The urea fluxes were typically increased from a basal rate of 2 to 10 and 25 nmol.cm(-2).min(-1) in the presence of vasopressin and forskolin, respectively. Flux activation consisted of a rapid-onset component of small amplitude that leveled off within approximately 10 min and at times even decreased again, followed by a delayed, strong increase over the next 30-40 min. Forskolin activated urea transport through activation of adenylyl cyclase; dideoxyforskolin was inactive. Vasopressin activated urea transport only from the basolateral side and was blocked by OPC-31260, indicating that its action was mediated by basolateral V(2) receptors. In the presence of the phosphodiesterase inhibitor IBMX, vasopressin activated as strongly as forskolin. By itself, IBMX caused a slow increase over 50 min to approximately 5 nmol.cm(-2).min(-1). 8-Bromoadenosine 3',5'-cyclic monophosphate (8-BrcAMP; 300 microM) activated urea flux only when added basolaterally. IBMX augmented the activation by basolateral 8-BrcAMP. Urea flux activation by vasopressin and forskolin were on...Continue Reading

References

Jun 1, 1992·The American Journal of Physiology·S M WallM A Knepper
Jun 1, 1992·The American Journal of Physiology·A G Gillin, J M Sands
Nov 1, 1987·The American Journal of Physiology·J M SandsM A Knepper
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·K B SeamonJ W Daly
Aug 1, 1993·Endocrine Journal·S Ishikawa
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·S NielsenM A Knepper
Dec 15, 1996·The Journal of Clinical Investigation·C Serradeil-Le GalG Le Fur
Aug 5, 1998·The American Journal of Physiology·F G Smith, I Abu-Amarah
Aug 5, 1999·Kidney International·B Andersen-BeckhA Oksche
Feb 24, 2001·American Journal of Physiology. Renal Physiology·J M TerrisJ B Wade
Aug 15, 2001·American Journal of Physiology. Renal Physiology·S M BagnascoJ M Sands
Dec 12, 2001·American Journal of Physiology. Renal Physiology·Chi ZhangJanet D Klein
Feb 22, 2002·Journal of the American Society of Nephrology : JASN·Janet D KleinJeff M Sands
Mar 19, 2002·The Journal of Biological Chemistry·Nicolas Laroche-JoubertAlain Doucet
Sep 10, 2002·American Journal of Physiology. Renal Physiology·R A FentonC P Smith
Jan 14, 2003·Annual Review of Physiology·Jeff M Sands
Jan 30, 2004·American Journal of Physiology. Cell Physiology·Otto FröhlichRobert B Gunn
Apr 13, 2004·American Journal of Physiology. Renal Physiology·Gavin S StewartCraig P Smith
May 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Robert A FentonMark A Knepper
Jun 29, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Gregory M SpringettDavid R Spriggs
Apr 15, 2005·Journal of the American Society of Nephrology : JASN·Robert A FentonMark A Knepper

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Citations

May 12, 2010·American Journal of Physiology. Cell Physiology·Abinash C MistryOtto Fröhlich
Jun 12, 2009·American Journal of Physiology. Renal Physiology·Abinash C MistryOtto Fröhlich
Aug 22, 2008·American Journal of Physiology. Renal Physiology·Nathan W BlessingJanet D Klein
Sep 12, 2008·American Journal of Physiology. Renal Physiology·Guangping ChenJeff M Sands
Aug 8, 2009·Journal of the American Society of Nephrology : JASN·Yanhua WangJeff M Sands
Apr 12, 2012·Journal of the American Society of Nephrology : JASN·Chenjuan YaoA S Verkman
Apr 1, 2011·British Journal of Pharmacology·Gavin Stewart
Sep 24, 2013·Chemistry & Biology·Cristina Esteva-FontA S Verkman
Mar 15, 2018·Physiological Reviews·William G Robichaux, Xiaodong Cheng
Sep 9, 2006·Journal of the American Society of Nephrology : JASN·Janet D KleinJeff M Sands
Jan 26, 2007·Journal of the American Society of Nephrology : JASN·Robert A Fenton, Mark A Knepper
Jul 20, 2006·The Journal of Biological Chemistry·Guangping ChenJeff M Sands
Dec 7, 2006·American Journal of Physiology. Renal Physiology·Gavin S StewartCraig P Smith
Jan 4, 2008·American Journal of Physiology. Renal Physiology·Otto FröhlichJeff M Sands
Feb 13, 2009·American Journal of Physiology. Cell Physiology·Udo Hasler
Dec 30, 2011·American Journal of Physiology. Renal Physiology·Titilayo O IloriJanet D Klein
Nov 6, 2009·American Journal of Physiology. Renal Physiology·R A FentonM Rützler
May 21, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cristina Esteva-FontA S Verkman
Jan 5, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marc H LevinA S Verkman
Aug 13, 2008·Current Opinion in Nephrology and Hypertension·Robert A Fenton
Nov 8, 2014·American Journal of Physiology. Renal Physiology·Carol A HobanMitsi A Blount
Aug 10, 2007·American Journal of Physiology. Renal Physiology·Mitsi A BlountJeff M Sands
Oct 24, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Susumu HyodoYoko Yamaguchi
Dec 5, 2008·American Journal of Physiology. Renal Physiology·G S StewartCraig Smith
Jan 23, 2015·American Journal of Physiology. Renal Physiology·Rikke NørregaardReena Rao

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