Regulation of vitellogenin (vtg1) and estrogen receptor (er) gene expression in zebrafish (Danio rerio) following the administration of Cd²⁺ and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)

Chemosphere
Ying Ying Chen, King Ming Chan

Abstract

We evaluated the individual and joint estrogenic effects of cadmium (Cd(2+)) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in the zebrafish liver (ZFL) cell line, zebrafish embryo, larvae and the liver of adult zebrafish. The mRNA expression of vtg1 was inhibited by Cd(2+), but unaffected by TCDD in ZFL cells. Similar changes in the mRNA levels of ERα, ERβ1, ERβ2 and GPER (G protein coupled estrogen receptor) in ZFL cells were also observed. Deletion mutants of vtg1 gene promoters were constructed to investigate transcriptional regulation, and we found that all of the constructs failed to respond to TCDD or Cd(2+). However, after co-transfection with a vtg1 promoter-luciferase construct to the ERα, ERβ1, ERβ2 and GPER expression vectors, decreased luciferase activity was observed in the ERα co-transfection group after treatment with Cd(2+), suggesting that ERα participates in vtg1 transcriptional regulation and is affected by Cd(2+). Differences in the regulation of the mRNA levels of these genes were also observed between different developmental stages and between the livers of male and female zebrafish.

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Citations

Oct 16, 2018·Frontiers in Endocrinology·Yu ChenHaoran Lin
Mar 5, 2021·Biological Reviews of the Cambridge Philosophical Society·Marta Lombó, Paz Herráez
Jul 29, 2019·Ecotoxicology and Environmental Safety·Chu Wa MakKing Ming Chan
Aug 5, 2021·Chemosphere·Maiara VicentiniHelena Cristina Silva de Assis
Aug 1, 2021·Environmental Toxicology and Pharmacology·Pai LiuWen-Fei Dong
Nov 16, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·S SreelekshmiR Moses Inbaraj

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