Regulation of voltage- and ligand-gated currents in rat hippocampal progenitor cells in vitro

Journal of Neurobiology
D W SahF H Gage

Abstract

Growth medium and substrate regulate the proliferation and differentiation of neural progenitor cells in vitro and the expression of cell type-specific histochemical markers. An important question is whether neural progenitor cells exhibit voltage- and ligand-gated currents, features characteristic of neurons, and whether these currents are regulated differentially by growth conditions. Another issue of interest is whether passaged progenitor cells, after expansion with basic fibroblast growth factor (FGF-2), exhibit the same degree of plasticity as their primary counterparts, or whether they are more committed to a particular phenotype. In primary cultures of embryonic rat hippocampal progenitor cells, growth in proliferative conditions (FGF-2) was associated with low levels of sodium, calcium, N-methyl-D-aspartate (NMDA), and kainate currents compared with other growth conditions. After multiple passages in the continued presence of FGF-2, sodium, calcium, and NMDA, responses declined further; interestingly, kainate and gamma-aminobutyric acid (GABA) responses remained substantial. Moreover, the expression of functional channels and receptors in primary cultures of progenitor cells is up-regulated strongly by growth factors s...Continue Reading

References

Jan 1, 1978·Annual Review of Neuroscience·P H Patterson
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G MandelP Brehm
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D P YounkinD Pleasure
Sep 1, 1993·Journal of Neurobiology·D M TuretskyD W Choi
Mar 1, 1994·Journal of Neurophysiology·T R CumminsG G Haddad
Jan 1, 1994·Annual Review of Neuroscience·M Hollmann, S Heinemann
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J RayF H Gage
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·M HoshimaruF H Gage

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Citations

Jan 13, 2006·Cell and Tissue Research·Joseph F SanchezWilliam J Freed
Apr 7, 2000·Brain Research. Brain Research Reviews·R Linden
Apr 8, 1998·Journal of Neuroscience Methods·M S EvansG J Brewer
Apr 29, 1998·Brain Research. Developmental Brain Research·H Cui, R F Bulleit
Apr 21, 1999·Brain Research Bulletin·M A CollingsM S Evans
Jan 12, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Elke Muth-KöhneMichael Hollmann
Oct 8, 2011·PloS One·Fatima H LabeedLisa A Flanagan
Jan 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sanjay K MistryKathryn L Crossin
Oct 2, 2012·Stem Cells International·Julieta Aprea, Federico Calegari
Dec 4, 2003·Journal of Neuroscience Research·Philip H SchwartzHenry Klassen
Mar 30, 2004·Journal of Neuroscience Research·Phuong B TranRichard J Miller
Mar 11, 2000·The European Journal of Neuroscience·C Vicario-AbejónR D McKay
Jan 20, 2012·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Kazuya ToriumiToshitaka Nabeshima
Aug 26, 1998·Journal of Neurobiology·F H GageJ Ray
Mar 25, 2010·Journal of Cellular Physiology·Sze-Ying NgSuk-Ying Tsang
Dec 17, 2003·Journal of Neurochemistry·Jingli CaiKatsutoshi Furukawa
Aug 16, 2005·Stem Cells·Philip H SchwartzHenry Klassen
Mar 5, 2002·Nature·Henriette van PraagFred H Gage
Jan 21, 2006·Hippocampus·Juan Nacher, Bruce S McEwen
Mar 8, 2006·The European Journal of Neuroscience·Marcello D'AscenzoClaudio Grassi
Mar 12, 2013·Cellular and Molecular Life Sciences : CMLS·Silvia BassaniMaria Passafaro
Apr 28, 2017·Breast Cancer Research : BCR·Cecilia ChoyRahul Jandial

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