Regulation of water permeability in rabbit conjunctival epithelium by anisotonic conditions

American Journal of Physiology. Cell Physiology
Oscar A CandiaA C Zamudio

Abstract

Effects of unilateral exposure to anisotonic conditions on diffusional water permeability of the isolated rabbit conjunctiva were determined. A segment of the bulbar-palpebral conjunctiva was mounted between Ussing-type hemichambers under short-circuit conditions. Unidirectional water fluxes (J(dw)) were measured in either direction by adding (3)H(2)O to one hemichamber and sampling from the other. Electrical parameters were measured simultaneously. J(dw) were determined under control isosmotic conditions and after introduction of either hyper- or hypotonic solutions against the tear or stromal sides of the preparations. In each of these four separate conditions, the anisotonic medium produced an approximately 20-30% reduction in J(dw) across the tissue, with the exception that to obtain such reduction with increased tonicity from the stromal side (medium osmolality increased by adding sucrose), conditions presumptively inhibiting regulatory volume increase mechanisms (e.g., pretreatment with amiloride and bumetanide) were also required. All reductions in J(dw) elicited by the various anisotonic conditions were reversible on restoration of control tonicity. In experiments in which preparations were pretreated with the protein c...Continue Reading

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Citations

Apr 25, 2007·Current Eye Research·Heng Zhu, Anuj Chauhan
Jun 1, 2007·American Journal of Physiology. Cell Physiology·R GeromettaO A Candia
Mar 14, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Rinki RayDaniel R Meldrum
Apr 4, 2008·American Journal of Physiology. Cell Physiology·Aldo C ZamudioRosana Gerometta
Jun 28, 2007·American Journal of Physiology. Endocrinology and Metabolism·Tim LahmDaniel R Meldrum

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