Regulation of WRN helicase activity in human base excision repair.

The Journal of Biological Chemistry
Byungchan AhnV A Bohr

Abstract

Werner syndrome patients are deficient in the Werner protein (WRN), which is a multifunctional nuclear protein possessing 3'-5' exonuclease and ATP-dependent helicase activities. Studies of Werner syndrome cells and biochemical studies of WRN suggest that WRN plays a role in several DNA metabolic pathways. WRN interacts with DNA polymerase beta (pol beta) and stimulates pol beta strand displacement synthesis on a base excision repair (BER) intermediate in a helicase-dependent manner. In this report, we examined the effect of the major human apurinic/apyrimidinic endonuclease (APE1) and of pol beta on WRN helicase activity. The results show that WRN alone is able to unwind several single strand break BER intermediates. However, APE1 inhibits WRN helicase activity on these intermediates. This inhibition is likely due to the binding of APE1 to nicked apurinic/apyrimidinic sites, suggesting that APE1 prevents the promiscuous unwinding of BER intermediates. This inhibitory effect was relieved by the presence of pol beta. A model involving the pol beta-mediated hand-off of WRN protein is proposed based on these results.

References

Apr 19, 1996·The Journal of Biological Chemistry·G FrosinaE Dogliotti
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·R A BennettB Demple
Jun 17, 1998·The Journal of Biological Chemistry·R PrasadS H Wilson
Nov 7, 1998·The Journal of Biological Chemistry·Y MasudaB Demple
Mar 4, 2000·Nature Structural Biology·S H Wilson, T A Kunkel
Oct 4, 2000·Nature Structural Biology·K S WilsonY Nakamura
Jul 4, 2001·Nucleic Acids Research·P MohagheghI D Hickson
Jul 31, 2001·The Journal of Biological Chemistry·S M YannoneD J Chen
Sep 14, 2001·Progress in Nucleic Acid Research and Molecular Biology·Y Matsumoto
Dec 18, 2001·Environmental and Molecular Mutagenesis·V A BohrR M Brosh
Mar 29, 2002·The Journal of Biological Chemistry·Cayetano von KobbeVilhelm A Bohr
Apr 9, 2002·Nucleic Acids Research·Bahrad A SokhansanjDavid M Wilson
Apr 17, 2002·The Journal of Biological Chemistry·Robert M BroshJoshua A Sommers
Apr 30, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Martin PootPeter S Rabinovitch
Aug 30, 2002·The Journal of Biological Chemistry·Tamara A RanalliRobert A Bambara
Sep 21, 2002·Molecular and Cellular Biology·Yannick SaintignyRaymond J Monnat
Oct 3, 2002·Biochemistry·Robert M BroshJoshua A Sommers
Apr 1, 2003·The Journal of Biological Chemistry·Jeanine A HarriganVilhelm A Bohr
Aug 26, 2003·Molecular Biology of the Cell·Juneth Joaquin PartridgeFred Eliezer Indig
Oct 10, 2003·The Journal of Biological Chemistry·Cayetano von KobbeVilhelm A Bohr
Mar 17, 2004·The Journal of Biological Chemistry·Patricia L OpreskoVilhelm A Bohr
Mar 24, 2004·Journal of Structural Biology·Fred Eliezer IndigVilhelm A Bohr
Jul 21, 2004·Molecular Cell·Lee WiederholdTapas K Hazra

❮ Previous
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Citations

Jul 31, 2007·Cellular and Molecular Life Sciences : CMLS·L S Cox, R G A Faragher
Dec 8, 2005·Biogerontology·Giovanni PaganoSeiji Kodama
Jun 11, 2005·Mutation Research·Ali Ozgenc, Lawrence A Loeb
May 27, 2005·Mutation Research·Vilhelm A Bohr
Sep 14, 2012·Molecular Biology of the Cell·Takashi TadokoroVilhelm A Bohr
Jul 2, 2009·Human Molecular Genetics·Shepherd H SchurmanVilhelm A Bohr
Feb 2, 2006·Nucleic Acids Research·Jeanine A HarriganVilhelm A Bohr
Nov 17, 2007·Nucleic Acids Research·Robert M Brosh, Vilhelm A Bohr
Oct 12, 2010·Nucleic Acids Research·Li ZhengBinghui Shen
May 17, 2011·Biochemistry. Biokhimii︠a︡·G L DianovJ L Parsons
Mar 13, 2014·Annual Review of Biochemistry·Deborah L CroteauVilhelm A Bohr
Aug 30, 2014·Nucleic Acids Research·Jolanta CzerwińskaElżbieta Speina
May 10, 2011·Progress in Neurobiology·Dennis Kjølhede JeppesenTinna Stevnsner
Oct 20, 2010·Seminars in Cancer Biology·Antonia A NemecJoann B Sweasy
Oct 12, 2010·Seminars in Cancer Biology·Raymond J Monnat
Mar 30, 2010·DNA Repair·Venkateswarlu PopuriVilhelm A Bohr
Apr 24, 2008·Mechanisms of Ageing and Development·Karen J OuyangNathan A Ellis
Dec 7, 2007·Free Radical Biology & Medicine·Ana LloretGiovanni Pagano
Jun 26, 2007·Experimental Gerontology·Wen-Hsing ChengVilhelm A Bohr
Sep 27, 2014·Biochimica Et Biophysica Acta·Jyotirindra MaityParimal Karmakar
Nov 23, 2006·Mechanisms of Ageing and Development·Rika KusumotoVilhelm A Bohr
May 5, 2006·Human Mutation·Shurong HuangJunko Oshima
Oct 10, 2015·BMC Medical Genetics·John A Smestad, L James Maher
Apr 19, 2016·DNA Repair·Satya NarayanRobert A Hromas
Jan 4, 2015·Ageing Research Reviews·Sebastian Veith, Aswin Mangerich
Jan 16, 2010·DNA Repair·Marie L RossiVilhelm A Bohr
May 1, 2016·Methods : a Companion to Methods in Enzymology·Piri WelcshJulia Sidorova
Jun 24, 2006·Methods in Enzymology·Robert M BroshVilhelm A Bohr
Jan 4, 2007·The Journal of Pathology·L Baumann
Jun 15, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Binghui ShenSteve Alas
Aug 24, 2006·The Biochemical Journal·Sudha SharmaRobert M Brosh
Jun 22, 2006·The Journal of Biological Chemistry·Kei-ichi TakataRichard D Wood
May 15, 2020·Proceedings of the National Academy of Sciences of the United States of America·Shrabasti RoychoudhuryKishor K Bhakat
Jul 6, 2007·The Journal of Biological Chemistry·Aditi DasVilhelm A Bohr
Jul 21, 2005·The Journal of Biological Chemistry·Patricia L OpreskoVilhelm A Bohr

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