Regulation of YKL-40 production by human articular chondrocytes

Arthritis and Rheumatism
J S JohansenM Lotz

Abstract

YKL-40 (human cartilage glycoprotein 39) is one of the most abundant proteins secreted by cultured chondrocytes. The objectives of the present study were to identify regulators of YKL-40 production in cartilage and chondrocytes and to map the localization of YKL-40 in chondrocytes. Human articular chondrocytes and cartilage explants (obtained from subjects at autopsy, from a tissue bank, and from osteoarthritis [OA] patients undergoing total joint replacement surgery) were stimulated with cytokines, growth factors, and other agents. YKL-40 expression was analyzed by Northern blot and polymerase chain reaction. YKL-40 secretion into the media was determined by enzyme-linked immunosorbent assay. YKL-40 production increased to very high levels during the early phase of chondrocyte monolayer culture and in normal cartilage explant cultures as a response to tissue injury. Spontaneous YKL-40 release was higher in OA than in normal cartilage explant cultures. In chondrocyte monolayer cultures, interleukin-1beta (IL-1beta) and transforming growth factor beta (TGFbeta) decreased the levels of secreted YKL-40, and this was associated with a reduction in YKL-40 messenger RNA levels. IL-1beta, but not TGFbeta, reduced YKL-40 production in ...Continue Reading

References

Apr 1, 1992·Matrix : Collagen and Related Research·H J HäuselmannE J Thonar
Jan 1, 1995·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J S JohansenP A Price
Nov 1, 1993·British Journal of Rheumatology·J S JohansenP A Price
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·A Varki
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·M F Meyer, G Kreil
Jun 1, 1997·Arthritis and Rheumatism·G F VerheijdenA M Boots
Jul 1, 1997·Osteoarthritis and Cartilage·M YoshiokaD Amiel
Jun 10, 1998·Journal of Cellular Biochemistry·F De CeuninckP M Vanhoutte
Apr 3, 1999·British Journal of Cancer·C CintinH J Nielsen
Jun 25, 1999·Scandinavian Journal of Rheumatology·B VolckP A Price
Jul 18, 2000·Journal of Hepatology·J S JohansenF Bendtsen

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Citations

Mar 25, 2009·International Orthopaedics·Sandra ZivanovićDusan Vucetić
Jul 27, 2010·American Journal of Respiratory Cell and Molecular Biology·Hiroshi MatsuuraChun Geun Lee
Oct 2, 2009·Future Oncology·Julia S JohansenBenny V Jensen
Aug 18, 2010·Marine Drugs·Feisal Khoushab, Montarop Yamabhai
Nov 13, 2008·Critical Reviews in Clinical Laboratory Sciences·Frederick D Coffman
Dec 1, 2001·Scandinavian Journal of Rheumatology·J S JohansenM Sharif
Jun 24, 2008·Ultrastructural Pathology·Anne RoslindThomas Horn
Dec 15, 2015·Brain and Behavior·Wenpeng LiuYuqing Zhang
Mar 30, 2010·Atherosclerosis·Annika E MichelsenBente Halvorsen
Aug 19, 2014·Mediators of Inflammation·Tuija VäänänenKatriina Vuolteenaho
Jan 18, 2007·Expert Opinion on Therapeutic Targets·Julia S JohansenPaul A Price
Aug 23, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Julia S JohansenKjeld Møllgård
Feb 14, 2009·The Journal of International Medical Research·K Huang, L D Wu
Mar 14, 2017·International Journal of Molecular Sciences·Lich Thi NguyenSang-Soo Lee
Apr 15, 2005·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Nanna JunkerPaul E G Kristjansen
Dec 18, 2008·International Journal of Cancer. Journal International Du Cancer·Anne K MylinLene M Knudsen
Jan 24, 2007·Journal of Molecular Histology·Merete RingsholtLise H Christensen
Jul 14, 2007·Coronary Artery Disease·Mine KucurSedat Tavsanoglu
Mar 13, 2010·Allergy, Asthma & Immunology Research·Chun Geun Lee, Jack A Elias
Feb 6, 2010·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Susanne Mayer-WagnerJörg Hausdorf
Oct 20, 2005·The Journal of Biological Chemistry·Anneliese D ReckliesSuzanne M Bernier
Dec 20, 2007·Applied Immunohistochemistry & Molecular Morphology : AIMM·Anne RoslindEva Balslev
Sep 16, 2020·Signal Transduction and Targeted Therapy·Ting ZhaoQiang You
Mar 18, 2020·Animals : an Open Access Journal From MDPI·Rong FuXiquan Zhang
Feb 24, 2006·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Julia S JohansenPaul A Price
May 4, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Christopher E PelloskiKenneth D Aldape

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