Regulators of floral fragrance production and their target genes in petunia are not exclusively active in the epidermal cells of petals.

Journal of Experimental Botany
Alex Van MoerkerckeRobert C Schuurink

Abstract

In which cells of the flower volatile biosynthesis takes place is unclear. In rose and snapdragon, some enzymes of the volatile phenylpropanoid/benzenoid pathway have been shown to be present in the epidermal cells of petals. It is therefore generally believed that the production of these compounds occurs in these cells. However, whether the entire pathway is active in these cells and whether it is exclusively active in these cells remains to be proven. Cell-specific transcription factors activating these genes will determine in which cells they are expressed. In petunia, the transcription factor EMISSION OF BENZENOIDS II (EOBII) activates the ODORANT1 (ODO1) promoter and the promoter of the biosynthetic gene isoeugenol synthase (IGS). The regulator ODO1 in turn activates the promoter of the shikimate gene 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). Here the identification of a new target gene of ODO1, encoding an ABC transporter localized on the plasma membrane, PhABCG1, which is co-expressed with ODO1, is described. PhABCG1 expression is up-regulated in petals overexpressing ODO1 through activation of the PhABCG1 promoter. Interestingly, the ODO1, PhABCG1, and IGS promoters were active in petunia protoplasts originat...Continue Reading

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Citations

Jul 1, 2015·Proceedings of the National Academy of Sciences of the United States of America·Myles P FenskeTakato Imaizumi
Dec 15, 2015·Phytochemistry·Timothy S JohnsonThomas A Colquhoun
Feb 7, 2013·The New Phytologist·Natalia DudarevaIan Kaplan
Jul 21, 2015·Trends in Plant Science·Joshua R WidhalmNatalia Dudareva
Mar 5, 2014·Plant, Cell & Environment·Joëlle K MuhlemannNatalia Dudareva
May 6, 2017·Frontiers in Plant Science·Ali NomanHe Shuilin
Feb 22, 2017·Frontiers in Plant Science·Reyhaneh Rahnamaie-TajadodNormah M Noor
Feb 9, 2020·The Journal of Experimental Biology·Adrian Brückner, Joseph Parker
Nov 23, 2017·Frontiers in Plant Science·Alon Cna'aniAlexander Vainstein
Apr 11, 2021·The Plant Journal : for Cell and Molecular Biology·Oded SkaliterAlexander Vainstein
Dec 23, 2016·Journal of Agricultural and Food Chemistry·Ross G Atkinson
Dec 5, 2021·The Plant Journal : for Cell and Molecular Biology·Maaike R BoersmaRobert C Schuurink

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Methods Mentioned

BETA
PCR
RNA blot
transgenic
Confocal microscopy
laser capture microdissection

Software Mentioned

CLUSTALW
ImageJ
BLAST

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