Regulators of G-protein signalling in Aspergillus nidulans: RgsA downregulates stress response and stimulates asexual sporulation through attenuation of GanB (Galpha) signalling

Molecular Microbiology
Kap-Hoon HanJae-Hyuk Yu

Abstract

Regulators of G-protein signalling play a crucial role in controlling the degree of heterotrimeric G-protein signalling. In addition to the previously studied flbA, we have identified three genes (rgsA, rgsB and rgsC) encoding putative RGS proteins in the genome of Aspergillus nidulans. Characterization of the rgsA gene revealed that RgsA downregulates pigment production and conidial germination, but stimulates asexual sporulation (conidiation). Deletion of rgsA (DeltargsA) resulted in reduced colony size with increased aerial hyphae, elevated accumulation of brown pigments as well as enhanced tolerance of conidia and vegetative hyphae against oxidative and thermal stress. Moreover, DeltargsA resulted in conidial germination in the absence of a carbon source. Deletion of both flbA and rgsA resulted in an additive phenotype, suggesting that the G-protein pathways controlled by FlbA and RgsA are different. Morphological and metabolic alterations caused by DeltargsA were suppressed by deletion of ganB encoding a Galpha subunit, indicating that the primary role of RgsA is to control negatively GanB-mediated signalling. Overexpression of rgsA caused inappropriate conidiation in liquid submerged culture, supporting the idea that GanB...Continue Reading

References

Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M M YeltonW E Timberlake
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·A P Schmitt, K McEntee
Aug 11, 1997·The Journal of Cell Biology·H A Fisk, M P Yaffe
Jan 26, 1999·Fungal Genetics and Biology : FG & B·M Bölker
Nov 5, 1999·Trends in Biochemical Sciences·B ZhengM Gist Farquhar
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·L De VriesM G Farquhar
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·K B LengelerJ Heitman
Mar 5, 2002·The Journal of Biological Chemistry·Eric N Johnson, Kirk M Druey
Mar 29, 2002·Molecular Microbiology·Kap-Hoon Han, Rolf A Prade
Jun 1, 2002·Science·Susana R NevesRavi Iyengar
Jul 9, 2002·Molecular Microbiology·Rukhsana HasanMichel B Toledano
Sep 12, 2002·Pharmacological Reviews·Susanne Hollinger, John R Hepler
Mar 7, 2003·Fungal Genetics and Biology : FG & B·Kim LangfelderAxel A Brakhage
May 31, 2003·Receptors & Channels·Peter Chidiac, Anju A Roy
Jan 1, 1953·Advances in Genetics·G PONTECORVOA W J BUFTON

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Citations

Aug 7, 2013·Applied Microbiology and Biotechnology·Yunchuan WangJinkui Yang
Oct 5, 2013·Applied Microbiology and Biotechnology·Susanne Nieland, K-Peter Stahmann
Nov 1, 2008·Applied Biochemistry and Biotechnology·Tamás EmriIstván Pócsi
Apr 16, 2005·Current Opinion in Biotechnology·Gertien J Smits, Stanley Brul
Oct 13, 2006·Eukaryotic Cell·Jae-Hyuk YuJeong-Ah Seo
May 18, 2007·Annual Review of Microbiology·Liande LiKatherine A Borkovich
Aug 5, 2005·Annual Review of Phytopathology·Jae-Hyuk Yu, Nancy Keller
Dec 13, 2005·BMC Genomics·Natalie D FedorovaWilliam C Nierman
Mar 4, 2014·PloS One·Hee-Soo ParkJae-Hyuk Yu
May 29, 2012·Fungal Genetics and Biology : FG & B·Ae Ran ParkYin-Won Lee
Jul 1, 2006·Molecular Plant Pathology·Marion Brodhagen, Nancy P Keller
Jul 23, 2011·Journal of Basic Microbiology·Nikoletta HegedusIstván Pócsi
Oct 7, 2004·Fungal Genetics and Biology : FG & B·Jae-Hyuk YuClaudio Scazzocchio
Nov 17, 2012·Microbiology·Melinda SzilágyiTamás Emri
Sep 25, 2008·FEMS Microbiology Reviews·Chaoyang XueJoseph Heitman
Jun 11, 2011·FEMS Microbiology Reviews·Ozgür Bayram, Gerhard H Braus
Jul 4, 2008·Acta Microbiologica Et Immunologica Hungarica·Tamás EmriIstván Pócsi
Mar 2, 2013·Studies in Mycology·P KrijgsheldH A B Wösten

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