Regulators of GTP-binding proteins cause morphological changes in the vacuole system of the filamentous fungus, Pisolithus tinctorius

Cell Motility and the Cytoskeleton
G J HydeA E Ashford

Abstract

Tubule formation is a widespread feature of the endomembrane system of eukaryotic cells, serving as an alternative to the better-known transport process of vesicular shuttling. In filamentous fungi, tubule formation by vacuoles is particularly pronounced, but little is known of its regulation. Using the hyphae of the basidiomycete Pisolithus tinctorius as our test system, we have investigated the effects of four drugs whose modulation, in animal cells, of the tubule/vesicle equilibrium is believed to be due to the altered activity of a GTP-binding protein (GTP gamma S, GDP beta S, aluminium fluoride, and Brefeldin A). In Pisolithus tinctorius, GTP gamma S, a non-hydrolysable form of GTP, strongly promoted vacuolar tubule formation in the tip cell and next four cells. The effects of GTP gamma S could be antagonised by pre-treatment of hyphae with GDP beta S, a non-phosphorylatable form of GDP. These results support the idea that a GTP-binding protein plays a regulatory role in vacuolar tubule formation. This could be a dynamin-like GTP-ase, since GTP gamma S-stimulated tubule formation has only been reported previously in cases where a dynamin is involved. Treatment with aluminium fluoride stimulated vacuolar tubule formation at...Continue Reading

References

Mar 1, 1992·The Journal of Cell Biology·R D KlausnerJ Lippincott-Schwartz
Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
Oct 1, 1986·The Journal of Cell Biology·M TerasakiK Fujiwara
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J SwansonS C Silverstein
May 1, 1987·The Journal of Cell Biology·J SwansonS C Silverstein
May 1, 1996·The Journal of Cell Biology·J R Henley, M A McNiven
Jul 1, 1996·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·R G Piña, C Cervantes
Feb 1, 1996·Journal of Microscopy·B Satiat-JeunemaitreC Hawes
May 26, 1997·FEBS Letters·M R AhmadianA Wittinghofer
Aug 11, 1997·The Journal of Cell Biology·A A MironovA Luini
May 8, 1998·Biochemical and Biophysical Research Communications·P J Padfield, N Panesar
Aug 26, 1998·Biochimica Et Biophysica Acta·J L Stow, K Heimann
Aug 28, 1998·Current Opinion in Cell Biology·S L SchmidP De Camilli
Aug 17, 1999·Current Opinion in Cell Biology·F Wieland, C Harter
Jan 5, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J R HenleyM A McNiven
Jan 12, 2001·Physiological Reviews·Y TakaiT Matozaki

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Citations

Feb 10, 2006·Eukaryotic Cell·Jun-ya ShojiKatsuhiko Kitamoto
Jan 10, 2008·Biotechnology and Bioengineering·Judith K PollackMark R Marten
Aug 21, 2012·Journal of Microbiological Methods·Andrea RichardsVeronica Veses
May 23, 2007·FEMS Microbiology Letters·Jun-ichi Maruyama, Katsuhiko Kitamoto
Sep 25, 2020·Scientific Reports·Joachim PfisterClemens Decristoforo
Apr 8, 2021·Molecular Biology of the Cell·Dale E LingoStephen A Osmani

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