Regulators of the protein phosphatase PP1γ2, PPP1R2, PPP1R7, and PPP1R11 are involved in epididymal sperm maturation

Journal of Cellular Physiology
Suranjana GoswamiSrinivasan Vijayaraghavan

Abstract

The serine/threonine protein phosphatase 1 (PP1) inhibitors PPP1R2, PPP1R7, and PPP1R11 are evolutionarily ancient and highly conserved proteins. Four PP1 isoforms, PP1α, PP1β, PP1γ1, and PP1γ2, exist; three of them except PP1γ2 are ubiquitous. The fact that PP1γ2 isoform is present only in mammalian testis and sperm led to the notion that isoform-specific regulators for PP1γ2 in sperm may be responsible for its function. In this report, we studied these inhibitors, PPP1R2, R7, and R11, to determine their spatial and temporal expression in testis and their regulatory functions in sperm. We show that, similar to PP1γ2, the three inhibitors are expressed at high levels in developing spermatogenic cells. However, the transcripts for the regulators are expressed as unique sizes in testis compared with somatic tissues. The three regulators share localization with PP1γ2 in the head and the principal piece of sperm. We show that the association of inhibitors to PP1γ2 changes during epididymal sperm maturation. In immotile caput epididymal sperm, PPP1R2 and PPP1R7 are not bound to PP1γ2, whereas in motile caudal sperm, all three inhibitors are bound as heterodimers or heterotrimers. In caudal sperm from male mice lacking sAC and glycog...Continue Reading

References

Nov 15, 1976·European Journal of Biochemistry·F L Huang, W H Glinsmann
Aug 31, 1990·Biochemical and Biophysical Research Communications·Y KitagawaM Nagao
Jul 30, 1993·Biochemical and Biophysical Research Communications·H ShimaM Nagao
Mar 1, 1996·Biology of Reproduction·G D SmithS Vijayaraghavan
Feb 9, 2000·Chemistry & Biology·J B AggenR Chamberlin
Jul 13, 2000·Biochemical and Biophysical Research Communications·Y S ChunM H Chung
Apr 12, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Hugo CeulemansMathieu Bollen
Nov 22, 2002·Biology of Reproduction·Zaohua HuangSrinivasan Vijayaraghavan
Jan 1, 1993·Current Biology : CB·E M StoneM Yanagida
Dec 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Seiji KawaTadashi Yamamoto
Dec 13, 2006·Nature Cell Biology·Shrikrishna DadkeJonathan Chernoff
Feb 16, 2007·Biology of Reproduction·Rumela ChakrabartiSrinivasan Vijayaraghavan
Mar 8, 2007·Molecular Reproduction and Development·Y B HanC J Haines
Jun 26, 2007·Asian Journal of Andrology·Rumela ChakrabartiSrinivasan Vijayaraghavan
Feb 27, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Tatsuya SuzukiMitsuaki Suzuki
Apr 20, 2010·Trends in Biochemical Sciences·Mathieu BollenMonique Beullens
Oct 14, 2010·Current Medicinal Chemistry·M FardilhaF F da Cruz e Silva
Jan 25, 2011·Molecular Human Reproduction·Margarida FardilhaEdgar da Cruz E Silva
Mar 1, 2012·The FEBS Journal·Ewald HeroesMathieu Bollen
Mar 20, 2013·BMC Cell Biology·Luis Korrodi-GregórioEdgar F da Cruz E Silva
Jul 31, 2013·Omics : a Journal of Integrative Biology·Margarida FardilhaEdgar F da Cruz e Silva
Nov 8, 2013·BMC Evolutionary Biology·Luís Korrodi-GregórioPedro J Esteves
Aug 5, 2014·Translational Research : the Journal of Laboratory and Clinical Medicine·Luís Korrodi-GregórioMargarida Fardilha
Jan 27, 2015·Current Molecular Pharmacology·Joana V SilvaMargarida Fardilha
Feb 1, 2018·Biology of Reproduction·Rahul BhattacharjeeSrinivasan Vijayaraghavan

❮ Previous
Next ❯

Citations

Sep 25, 2019·Proceedings of the National Academy of Sciences of the United States of America·Meng S ChoyRebecca Page
Jan 10, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Souvik DeySrinivasan Vijayaraghavan
Jan 11, 2020·Frontiers in Cell and Developmental Biology·Souvik DeySrinivasan Vijayaraghavan
Nov 17, 2020·Systems Biology in Reproductive Medicine·Sellappan SelvarajuMuniandy Sivaram
Oct 21, 2020·Fertility and Sterility·Ettore Caroppo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.