Regulatory Architecture of the LβT2 Gonadotrope Cell Underlying the Response to Gonadotropin-Releasing Hormone

Frontiers in Endocrinology
Frederique Ruf-ZamojskiStuart C Sealfon

Abstract

The LβT2 mouse pituitary cell line has many characteristics of a mature gonadotrope and is a widely used model system for studying the developmental processes and the response to gonadotropin-releasing hormone (GnRH). The global epigenetic landscape, which contributes to cell-specific gene regulatory mechanisms, and the single-cell transcriptome response variation of LβT2 cells have not been previously investigated. Here, we integrate the transcriptome and genome-wide chromatin accessibility state of LβT2 cells during GnRH stimulation. In addition, we examine cell-to-cell variability in the transcriptional response to GnRH using Gel bead-in-Emulsion Drop-seq technology. Analysis of a bulk RNA-seq data set obtained 45 min after exposure to either GnRH or vehicle identified 112 transcripts that were regulated >4-fold by GnRH (FDR < 0.05). The top regulated transcripts constitute, as determined by Bayesian massive public data integration analysis, a human pituitary-relevant coordinated gene program. Chromatin accessibility [assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq)] data sets generated from GnRH-treated LβT2 cells identified more than 58,000 open chromatin regions, some containing notche...Continue Reading

References

May 1, 1992·Endocrine Reviews·S S Stojilković, K J Catt
Oct 3, 2001·The Journal of Biological Chemistry·E WurmbachS C Sealfon
Jun 1, 2002·Molecular Endocrinology·Tony YuenStuart C Sealfon
Jun 12, 2002·Molecular Biology of the Cell·Michael L WhitfieldDavid Botstein
Oct 6, 2004·Genome Biology·Robert C GentlemanJianhua Zhang
Sep 24, 2005·Science·Jonathan M Raser, Erin K O'Shea
Dec 24, 2005·Nature·Dmitri VolfsonJeff Hasty
Feb 8, 2006·Journal of Molecular Endocrinology·Pankaj LambaDaniel J Bernard
Aug 19, 2006·The Journal of Biological Chemistry·Frederique RufStuart C Sealfon
Aug 28, 2007·Biophysical Journal·Frederique RufStuart C Sealfon
Nov 21, 2007·Molecular and Cellular Endocrinology·Magdalena I SuszkoTeresa K Woodruff
Sep 19, 2008·Genome Biology·Yong ZhangX Shirley Liu
Mar 11, 2009·Molecular and Cellular Biology·Nicole Y Tan, Levon M Khachigian
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
May 3, 2012·The Journal of Biological Chemistry·Soon-Gang ChoiStuart C Sealfon
Oct 30, 2012·Bioinformatics·Alexander DobinThomas R Gingeras
Dec 6, 2012·Molecular Endocrinology·Qian WangStuart C Sealfon
Aug 13, 2013·PLoS Computational Biology·C J ZopfNarendra Maheshri
Sep 3, 2013·Molecular and Cellular Endocrinology·Hanna PincasStuart C Sealfon
Feb 4, 2014·Genome Biology·Charity W LawGordon K Smyth
Jan 7, 2015·Current Protocols in Molecular Biology·Jason D BuenrostroWilliam J Greenleaf
Jan 22, 2015·Nucleic Acids Research·Matthew E RitchieGordon K Smyth
Apr 29, 2015·Nature Genetics·Casey S GreeneOlga G Troyanskaya

❮ Previous
Next ❯

Citations

Dec 6, 2018·Endocrinology·George A StamatiadesUrsula B Kaiser
Apr 26, 2019·Journal of the Endocrine Society·Frederique Ruf-ZamojskiStuart C Sealfon
Oct 18, 2019·Frontiers in Endocrinology·Patrick A FletcherStanko S Stojilkovic
Jun 11, 2021·Endocrinology·Tal Refael, Philippa Melamed

❮ Previous
Next ❯

Methods Mentioned

BETA
RNA-seq
Drop-seq
Assay
PCR
ATAC-seq
chip

Software Mentioned

Cell Ranger
Limma
Kapa
Ensembl
STAR
Bioconductor
Homologene
RSEM
R package cellrangerRkit
GEM Drop - seq

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.