Regulatory Compliant Tissue-Engineered Human Corneal Endothelial Grafts Restore Corneal Function of Rabbits with Bullous Keratopathy

Scientific Reports
Gary PehJodhbir S Mehta

Abstract

Corneal transplantation is the only treatment available to restore vision for individuals with blindness due to corneal endothelial dysfunction. However, severe shortage of available donor corneas remains a global challenge. Functional regulatory compliant tissue-engineered corneal endothelial graft substitute can alleviate this reliance on cadaveric corneal graft material. Here, isolated primary human corneal endothelial cells (CEnCs) propagated using a dual media approach refined towards regulatory compliance showed expression of markers indicative of the human corneal endothelium, and can be tissue-engineered onto thin corneal stromal carriers. Both cellular function and clinical adaptability was demonstrated in a pre-clinical rabbit model of bullous keratopathy using a tissue-engineered endothelial keratoplasty (TE-EK) approach, adapted from routine endothelial keratoplasty procedure for corneal transplantation in human patients. Cornea thickness of rabbits receiving TE-EK graft gradually reduced over the first two weeks, and completely recovered to a thickness of approximately 400 µm by the third week of transplantation, whereas corneas of control rabbits remained significantly thicker over 1,000 µm (p < 0.05) throughout t...Continue Reading

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Citations

Jun 13, 2020·Nature Reviews. Disease Primers·Yu Qiang SohJodhbir S Mehta
Nov 18, 2018·Scientific Reports·Kazuya YamashitaShigeto Shimmura
Sep 11, 2018·Translational Vision Science & Technology·Stephen WahligJodhbir S Mehta
Jun 4, 2019·Investigative Ophthalmology & Visual Science·Xin XiaJeffrey L Goldberg
May 6, 2020·Scientific Reports·Ricardo F FraustoAnthony J Aldave
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Jul 28, 2020·The British Journal of Ophthalmology·Hon Shing OngJodhbir Mehta
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Dec 31, 2021·Acta Ophthalmologica·Emmanuel CrouzetGilles Thuret

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Methods Mentioned

BETA
flow cytometry
chromosomal aberrations
chromosomal aberration
scanning electron microscopy
PCR
dissection
dissecting

Software Mentioned

TrypLE
Cryoscarless
Visante AS -
SPSS Statistics

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