Regulatory effect of monocytes on T cell proliferative responses to oral microbial antigens.

Infection and Immunity
P Stashenko

Abstract

Mononuclear cell preparations isolated by Ficoll-Hypaque centrifugation from human peripheral blood were found to vary considerably in the number of monocytes they contained (mean, 20.3%; range, 13 to 33%). The regulatory role of monocytes in T cell proliferative responses to sonic extracts of a panel of oral microorganisms was therefore investigated. T cells were fractionated by anti-immunoglobulin chromatography and depleted of monocytes by treatment with a monoclonal anti-human Ia-like (DR locus antigen) antibody and complement. Purified populations of monocytes were obtained by extensive adherence procedures. The resultant cell populations were greater than 95% pure, as judged by indirect immunofluorescence on a fluorescence-activated cell sorter. Monocyte-depleted T cells failed to respond by proliferation to the nonoral antigen tetanus toxoid, as well as to any oral microorganism, but retained responsiveness to phytohemagglutinin. Readdition of monocytes in final concentrations of from 5 to 15% resulted in the restoration of maximal T cell proliferation. Monocytes in greater numbers suppressed T cell responses to all sonic extracts tested.

References

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Citations

Dec 1, 1988·Journal of Periodontology·G M Newcomb, R N Powell
Oct 1, 1988·Journal of Periodontology·R A ReinhardtW B Kaldahl
Jul 1, 1989·Journal of Periodontal Research·J TewD Mangan
Mar 1, 1989·Journal of Periodontal Research·H H TopollC Sorg
Nov 1, 1983·Journal of Periodontal Research·P StashenkoS S Socransky
Sep 1, 1985·Journal of Periodontal Research·P StashenkoS S Socransky
Jun 1, 1990·Endodontics & Dental Traumatology·P Stashenko
Nov 1, 1996·Journal of Periodontal Research·M D Petit, P Stashenko
Nov 1, 1983·Infection and Immunity·S L DonaldsonJ G Tew

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