Regulatory effects of zinc and copper on the calcium transport system in rat liver nuclei. Relation to SH groups in the releasing mechanism

Biochemical Pharmacology
M Yamaguchi

Abstract

In isolated hepatic nuclei, the heavy metals Zn2+ and Cu2+ (10 microM) inhibited Ca2+ uptake and caused a prompt release of Ca2+ from preloaded nuclei in a concentration-dependent manner, with Zn2+ being more effective than Cu2+. The sulfhydryl group reducing agent dithiothreitol (DTT) protected the nuclei from the effects of heavy metals; DDT (1 mM) almost completely blocked Zn(2+)- or Cu(2+)-induced Ca2+ release and inhibition of Ca2+ uptake. The sulfhydryl modifying reagent N-ethylmaleimide (NEM; 0.2 mM) also caused Ca2+ release, but it did not have an appreciable effect on Ca2+ uptake. Furthermore, in the presence of NEM heavy metals did not evoke Ca2+ release. The present study demonstrates that Zn2+ and Cu2+ have a stimulatory effect on Ca2+ release from isolated rat liver nuclei, and that the SH group may play an important role in the Ca(2+)-releasing mechanism in liver nuclei.

References

Apr 1, 1990·Biochemical Medicine and Metabolic Biology·M Yamaguchi, S Mori
Jan 1, 1984·Physiological Reviews·N Kraus-Friedmann
Mar 1, 1983·Archives of Biochemistry and Biophysics·A Bindoli, S Fleischer
Dec 30, 1981·Biochimica Et Biophysica Acta·J R WilliamsonJ B Hoek
Jul 31, 1980·Biochemical and Biophysical Research Communications·A L BoyntonJ P MacManus

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Citations

Jun 1, 1994·Journal of Cellular Biochemistry·K Oishi, M Yamaguchi
Jul 5, 1995·Molecular and Cellular Biochemistry·M Yamaguchi, K Oishi
Oct 1, 1994·Pflügers Archiv : European journal of physiology·A A BendersJ H Veerkamp

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