Regulatory elements in the minimal promoter region of the mouse Xist gene

Gene
S A SheardownN Brockdorff

Abstract

The Xist gene plays a central role in regulating X chromosome inactivation and Xist transcription has recently been shown to be necessary for X inactivation in mouse. We are currently analysing regulation of the Xist gene in order to determine the mechanisms underlying initiation of Xist expression and X inactivation. Sequence comparisons indicate that a region of approximately 0.4 kb upstream of the the major transcriptional start site comprises the Xist minimal promoter. Analysis of reporter constructs demonstrates that the minimal promoter region is active both in embryonic stem (ES) cells and in differentiated derivatives, indicating that sequences either further upstream or downstream are required for appropriate developmental control of Xist transcription. We have examined the minimal promoter region in detail, and in addition to common promoter elements have identified two previously uncharacterised transcription-factor binding sites. Mutation of these sites in reporter constructs indicates that they are functionally important.

References

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Citations

Oct 12, 2000·Gene·L V Pereira, L R Vasques
Jan 14, 2003·Seminars in Cell & Developmental Biology·Philippe Clerc, Philip Avner
May 1, 2002·The EMBO Journal·Ludmila BozhenokPatrick Varga-Weisz
Mar 10, 2010·BMC Molecular Biology·Samuel C Chang, Carolyn J Brown
May 19, 2012·PloS One·Konstantin E OrishchenkoSuren M Zakian
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·V McCabeN Brockdorff
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·D WarshawskyJ T Lee
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·J T LeeY Han
Jun 30, 2005·Chromosoma·Cecile C de la CruzBarbara Panning
Aug 5, 1998·Current Opinion in Genetics & Development·N Brockdorff
Jul 5, 2005·Developmental Cell·Takashi SadoHiroyuki Sasaki
May 27, 2008·Developmental Biology·Chia-Lun TsaiJeannie T Lee
Oct 20, 2015·Nature Communications·Mrinal K SarkarSundeep Kalantry
Mar 31, 2011·Genetika·A M KorotkovaS M Zakiian
Nov 17, 2006·Development·Takashi SadoHiroyuki Sasaki

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