Regulatory elements in the promoter region of the rat gene encoding the acyl-CoA-binding protein

Gene
M ElholmS Mandrup

Abstract

Acyl-CoA-binding protein (ACBP) is an ubiquitously expressed 10-kDa protein which is present in high amounts in cells involved in solute transport or secretion. Rat ACBP is encoded by a gene containing the typical hallmarks of a housekeeping gene. Analysis of the promoter region of the rat ACBP gene by electrophoretic mobility shift assay (EMSA) revealed specific binding of proteins from rat liver nuclear extracts to potential recognition sequences of NF-1/CTF, Sp1, AP-1, C/EBP and HNF-3. In addition, specific binding to a DR-1 type element was observed. By using in vitro translated peroxisome proliferator activated receptors (PPAR) and a retinoid X receptor alpha (RXRalpha), we demonstrated that this DR-1 element was capable of binding PPARalpha/RXRalpha, PPARdelta/RXRalpha and PPARgamma2/RXRalpha heterodimers. The PPARgamma2/RXRalpha heterodimer appeared to have the highest affinity for the ACBP DR-1 element. Addition of peroxisome proliferators (PP) to H4IIEC3 rat hepatoma cells led to an increase in the ACBP mRNA level, indicating that the DR-1 element could be a functional peroxisome proliferator responsive element (PPRE). Analysis of the ACBP promoter by transient transfection showed that deletion of the region containing...Continue Reading

References

May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M GöttlicherJ A Gustafsson
May 11, 1991·Nucleic Acids Research·K JolliffL F Johnson
Mar 1, 1989·Molecular and Cellular Biology·T A Ryden, K Beemon
Mar 25, 1987·Nucleic Acids Research·J T den Dunnen, J G Schoenmakers
Nov 11, 1987·Nucleic Acids Research·J F MilliganO C Uhlenbeck
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M KolmerL Pani
Jan 1, 1994·Progress in Growth Factor Research
May 15, 1994·Genes & Development·P TontonozB M Spiegelman
Jun 9, 1993·Molecular and Cellular Biochemistry·J KnudsenK Kristiansen
Oct 1, 1993·Journal of Biochemistry·K SogawaY Fujii-Kuriyama
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K L GearingJ A Gustafsson
Dec 15, 1995·Cell·D J Mangelsdorf, R M Evans
Nov 1, 1993·Trends in Endocrinology and Metabolism : TEM·H Keller, W Wahli

❮ Previous
Next ❯

Citations

Mar 24, 2000·Progress in Lipid Research·B I Frohnert, D A Bernlohr
Oct 9, 2007·Bioinformatics·Bo JiangXuegong Zhang
Jan 7, 2005·Nutrition & Metabolism·Zahra Fatehi-Hassanabad, Catherine B Chan
Mar 5, 2004·Journal of Lipid Research·Klaus Stensgaard FrederiksenJan Fleckner
Oct 26, 1999·Endocrine Reviews·V Giguère
Jul 10, 2019·Pharmacology & Therapeutics·Marie-Christine TononJérôme Leprince

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.