PMID: 9171887Dec 1, 1996Paper

Regulatory elements of the mb-1 gene encoding the Ig-alpha component of the human B-cell antigen receptor

Molecular Immunology
I Leduc, M Cogne

Abstract

The mb-1 gene encodes the Ig-alpha component of the B-cell antigen receptor. It is specifically expressed in pre-B and mature B cells but not in plasma cells losing membrane Ig (mIg) expression. We looked for transcriptional regulatory elements within a 12 kb genomic fragment. A strong promoter activity was found in a 591 bp fragment harboring consensus binding sites for known transcription factors including Ets, EBF/BlyF, LyF1/micro B and Spl. It was able to drive transcription of a reporter gene in the absence of any additional enhancer and was mostly active in B lymphocytes not in plasma cells or T cells. Although no fragment from the mb-1 gene displayed enhancer activity in combination with either the SV40, a Ig VH or a Ig VL promoter, a 1078 bp fragment corresponding to the 5' part of the gene behaved as a strong enhancer in either orientation in constructs driven by the mb-1 promoter itself. Deletions within this fragment allowed to delineate shorter sequences with enhancer activity upstream the first exon. The tissue-restricted, promoter-restricted and stage-specific activity of this 5' flanking region suggests that it is the main regulatory element of the mb-1 gene.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T NakamuraM D Cooper
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J Hagman, R Grosschedl
May 1, 1992·Cell Growth & Differentiation : the Molecular Biology Journal of the American Association for Cancer Research·A SethT S Papas
Jan 25, 1992·Proceedings of the National Academy of Sciences of the United States of America·K IshiharaP W Kincade
Jun 1, 1991·Immunology Today·M RethJ C Cambier
Aug 29, 1991·Nature·A R VenkitaramanM S Neuberger
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S T SmaleD Baltimore
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G G HermansonR Wall
Apr 1, 1995·Current Opinion in Cell Biology·A L DeFranco
Jan 1, 1994·International Immunology·K JohanssonT Leanderson
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S A Omori, R Wall

❮ Previous
Next ❯

Citations

Nov 24, 2012·Leukemia·V BohleR Küppers
Apr 1, 2004·International Journal of Cancer. Journal International Du Cancer·Paula PerssonMikael Sigvardsson
Feb 15, 2001·Oncogene·V I Sementchenko, D K Watson
May 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ramiro Gisler, Mikael Sigvardsson

❮ Previous
Next ❯

Related Concepts

Related Feeds

B cell Differentiation

Depending on the signal received through the B cell receptor and other receptors, B cells differentiate into follicular or marginal zone B cells. Here is the latest research pertaining to this differentiation process.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.