PMID: 6974296Jan 1, 1981Paper

Regulatory factors of lymphocyte-lymphocyte interaction. I. Con A-induced mitogenic factor acts on the late G1 stage of T-cell proliferation

Microbiology and Immunology
M KawanoM Hanaoka

Abstract

DNA synthesis in murine lymphocytes was augmented by a soluble factor in the supernatant of serum-free cultures of syngeneic spleen cells activated with concanavalin A (Con A). This so-called mitogenic factor (MF), which is probably identical with interleukin II, partially purified by DEAE-cellulose and Sephadex G-75 chromatography, is a fairly homogeneous molecule of 17-25 X 10(3) daltons. By using partially purified MF, the role of MF in lymphocyte proliferation was investigated. Pretreatment of lymphocytes with Con A for the first 3 hr of culture, which does not in itself induce cell proliferation, markedly augmented the effect of MF. The presence of MF, however, is necessary only in a restricted stage(s) of lymphocyte proliferation. The addition and removal of MF at various times during culture showed that MF exerts its effect on a process which occurs 3-6 hr before the beginning of DNA synthesis. These results strongly suggest that MF regulates the proliferation of mitogen-stimulated lymphocytes by acting on a restricted stage(s) of the cell cycle.

References

Jan 1, 1976·Cellular Immunology·B H Waksman, Y Namba
Dec 2, 1976·Nature·S ToyoshimaT Osawa
Nov 1, 1973·Cellular Immunology·D A HartA Nisonoff
Nov 1, 1974·The Journal of Experimental Medicine·R E RocklinJ R David
Nov 1, 1973·Cellular Immunology·J M JonesA Nisonoff
Oct 4, 1969·Nature·R N MainiD C Dumonde

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Citations

Aug 15, 2015·Journal of Animal Science and Biotechnology·W T Oliver, J E Wells
Oct 1, 1985·Immunological Investigations·H KomadaK Izutsu
Mar 25, 2015·Journal of Applied Microbiology·J E WellsW T Oliver
Jul 1, 1983·Acta Oto-laryngologica·N YamanakaA Kataura
Dec 1, 2019·Probiotics and Antimicrobial Proteins·Deon P NevelingL M T Dicks
Jun 4, 2002·The Journal of Nutrition·Brooke D HumphreyKirk C Klasing
Aug 14, 2021·Analytical Biochemistry·Faezeh TaghaviSeyed Mohammad Taghdisi

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