Regulatory functions of serine-46-phosphorylated HPr in Lactococcus lactis

Journal of Bacteriology
Vicente MonederoJ Deutscher

Abstract

In most low-G+C gram-positive bacteria, the phosphoryl carrier protein HPr of the phosphoenolpyruvate:sugar phosphotransferase system (PTS) becomes phosphorylated at Ser-46. This ATP-dependent reaction is catalyzed by the bifunctional HPr kinase/P-Ser-HPr phosphatase. We found that serine-phosphorylated HPr (P-Ser-HPr) of Lactococcus lactis participates not only in carbon catabolite repression of an operon encoding a beta-glucoside-specific EII and a 6-P-beta-glucosidase but also in inducer exclusion of the non-PTS carbohydrates maltose and ribose. In a wild-type strain, transport of these non-PTS carbohydrates is strongly inhibited by the presence of glucose, whereas in a ptsH1 mutant, in which Ser-46 of HPr is replaced with an alanine, glucose had lost its inhibitory effect. In vitro experiments carried out with L. lactis vesicles had suggested that P-Ser-HPr is also implicated in inducer expulsion of nonmetabolizable homologues of PTS sugars, such as methyl beta-D-thiogalactoside (TMG) and 2-deoxy-D-glucose (2-DG). In vivo experiments with the ptsH1 mutant established that P-Ser-HPr is not necessary for inducer expulsion. Glucose-activated 2-DG expulsion occurred at similar rates in wild-type and ptsH1 mutant strains, wherea...Continue Reading

References

Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M J Weickert, G H Chambliss
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J Deutscher, M H Saier
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·J J YeM H Saier
Sep 1, 1993·Microbiological Reviews·P W PostmaG R Jacobson
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·A GalinierJ Haiech
Mar 14, 2000·Journal of Bacteriology·P L HuynhR Brückner
Oct 6, 2000·Annual Review of Microbiology·J Stülke, W Hillen
Sep 1, 1990·Applied and Environmental Microbiology·K J LeenhoutsG Venema

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Citations

Feb 14, 2009·Applied Microbiology and Biotechnology·Felix Lopez de Felipe, Philippe Gaudu
Jun 9, 2005·FEMS Microbiology Reviews·Ana Rute NevesHelena Santos
Mar 14, 2012·Antimicrobial Agents and Chemotherapy·Christina GabrielsenDzung B Diep
Oct 22, 2008·Journal of Bacteriology·Catalina Arango Pinedo, Daniel J Gage
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherPieter W Postma
Nov 11, 2008·Canadian Journal of Microbiology·Denis J RoyChristian Vadeboncoeur
Jan 18, 2006·Journal of Molecular Microbiology and Biotechnology·Rana HerroJosef Deutscher
Dec 22, 2006·Journal of Molecular Microbiology and Biotechnology·Vicente MonederoJosef Deutscher
Oct 20, 2005·Journal of Bioscience and Bioengineering·P KotrbaH Yukawa
Aug 21, 2013·PloS One·Xinglin ZhangWillem van Schaik
Oct 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ivan MijakovicJosef Deutscher
Apr 25, 2007·Critical Reviews in Microbiology·Magdalena Kowalczyk, Jacek Bardowski
Sep 27, 2005·Biochimica Et Biophysica Acta·Josef DeutscherSandrine Poncet
Dec 22, 2007·Research in Microbiology·Vicente MonederoJosef Deutscher
Sep 23, 2014·Applied Microbiology and Biotechnology·Tamara Aleksandrzak-PiekarczykJacek Bardowski
Nov 13, 2004·Proteomics·Johan PalmfeldtPeter James
Sep 26, 2003·Molecular Microbiology·Philippe GauduAlexandra Gruss

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