Regulatory interactions of a virulence-associated serine/threonine phosphatase-kinase pair in Bacillus anthracis.

Journal of Bacteriology
Salika M ShakirJimmy D Ballard

Abstract

In the current study, we examined the regulatory interactions of a serine/threonine phosphatase (BA-Stp1), serine/threonine kinase (BA-Stk1) pair in Bacillus anthracis. B. anthracis STPK101, a null mutant lacking BA-Stp1 and BA-Stk1, was impaired in its ability to survive within macrophages, and this correlated with an observed reduction in virulence in a mouse model of pulmonary anthrax. Biochemical analyses confirmed that BA-Stp1 is a PP2C phosphatase and dephosphorylates phosphoserine and phosphothreonine residues. Treatment of BA-Stk1 with BA-Stp1 altered BA-Stk1 kinase activity, indicating that the enzymatic function of BA-Stk1 can be influenced by BA-Stp1 dephosphorylation. Using a combination of mass spectrometry and mutagenesis approaches, three phosphorylated residues, T165, S173, and S214, in BA-Stk1 were identified as putative regulatory targets of BA-Stp1. Further analysis found that T165 and S173 were necessary for optimal substrate phosphorylation, while S214 was necessary for complete ATP hydrolysis, autophosphorylation, and substrate phosphorylation. These findings provide insight into a previously undescribed Stp/Stk pair in B. anthracis.

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Citations

Feb 12, 2011·The Journal of Biological Chemistry·Jiyong SuKarl Forchhammer
Feb 8, 2011·Journal of Bacteriology·Katie M Bryant-HudsonJimmy D Ballard
Mar 5, 2011·Microbiology and Molecular Biology Reviews : MMBR·Sandro F F PereiraJonathan Dworkin
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Jun 25, 2013·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Gunjan AroraYogendra Singh
Sep 12, 2015·Annual Review of Microbiology·Andaleeb SajidYogendra Singh
Jun 22, 2012·Enzyme Research·Jiyong Su, Karl Forchhammer
Jul 25, 2018·Journal of Bacteriology·Ja E ClaywellDerek J Fisher
Apr 27, 2016·Journal of Bacteriology·Ja E Claywell, Derek J Fisher
Mar 13, 2019·Journal of Bacteriology·Benjamin D LabbeChristopher J Kristich
Aug 16, 2017·Journal of Bacteriology·Benjamin D Labbe, Christopher J Kristich
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May 8, 2018·Frontiers in Microbiology·Weihao ZhengZigang Li
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Jul 25, 2017·Trends in Microbiology·Daniel A PensingerJohn-Demian Sauer
Sep 29, 2021·Chemical Biology & Drug Design·Ashley King, Meghan S Blackledge

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