Regulatory link between DNA methylation and active demethylation in Arabidopsis

Proceedings of the National Academy of Sciences of the United States of America
Mingguang LeiJian-Kang Zhu

Abstract

De novo DNA methylation through the RNA-directed DNA methylation (RdDM) pathway and active DNA demethylation play important roles in controlling genome-wide DNA methylation patterns in plants. Little is known about how cells manage the balance between DNA methylation and active demethylation activities. Here, we report the identification of a unique RdDM target sequence, where DNA methylation is required for maintaining proper active DNA demethylation of the Arabidopsis genome. In a genetic screen for cellular antisilencing factors, we isolated several REPRESSOR OF SILENCING 1 (ros1) mutant alleles, as well as many RdDM mutants, which showed drastically reduced ROS1 gene expression and, consequently, transcriptional silencing of two reporter genes. A helitron transposon element (TE) in the ROS1 gene promoter negatively controls ROS1 expression, whereas DNA methylation of an RdDM target sequence between ROS1 5' UTR and the promoter TE region antagonizes this helitron TE in regulating ROS1 expression. This RdDM target sequence is also targeted by ROS1, and defective DNA demethylation in loss-of-function ros1 mutant alleles causes DNA hypermethylation of this sequence and concomitantly causes increased ROS1 expression. Our results...Continue Reading

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Citations

Jan 8, 2016·Journal of Experimental Botany·Karolina ChwialkowskaMiroslaw Kwasniewski
Jul 6, 2016·Frontiers in Plant Science·Philippe GallusciGraham B Seymour
Aug 6, 2016·Annual Review of Phytopathology·A DelerisL Navarro
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Nov 4, 2016·Nature Plants·Tzung-Fu Hsieh
Jan 18, 2018·Proceedings of the National Academy of Sciences of the United States of America·Yu ZhangSteven E Jacobsen
Dec 28, 2018·Journal of Integrative Plant Biology·Xinlong XiaoMingguang Lei
Oct 29, 2017·Physiologia Plantarum·Maria Luz AnnacondiaGerman Martinez
Jul 20, 2019·Biochemical Society Transactions·Huiming ZhangJian-Kang Zhu
Oct 28, 2017·The Plant Journal : for Cell and Molecular Biology·Dolores Córdoba-CañeroJean Molinier
Oct 20, 2019·Journal of Integrative Plant Biology·Ruie Liu, Zhaobo Lang
Sep 26, 2019·Molecular Biology and Evolution·Simone FouchéDaniel Croll
Mar 18, 2020·G3 : Genes - Genomes - Genetics·Stefania VendraminKaren M McGinnis
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Aug 20, 2020·Plant Molecular Biology·Melisa LeoneSebastián Asurmendi
Jul 28, 2020·The Plant Journal : for Cell and Molecular Biology·Dae Kwan Ko, Federica Brandizzi
Jul 26, 2018·International Journal of Molecular Sciences·Arthur BartelsWenyan Xiao
Sep 30, 2018·Proceedings of the National Academy of Sciences of the United States of America·Haifeng ZhuJian-Kang Zhu
Jun 20, 2019·Frontiers in Plant Science·Daniel KuźnickiJolanta Floryszak-Wieczorek
Jun 14, 2019·Epigenetics : Official Journal of the DNA Methylation Society·Ulrike SchumannMing-Bo Wang
Aug 1, 2019·Proceedings of the National Academy of Sciences of the United States of America·Wen-Feng NieJian-Kang Zhu

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