Regulatory mechanism for expression of IL1B receptors in the uterine endometrium and effects of IL1B on prostaglandin synthetic enzymes during the implantation period in pigs

Biology of Reproduction
Heewon SeoHakhyun Ka

Abstract

During the implantation period, the porcine conceptus secretes interleukin-1beta (IL1B) that may be involved in the establishment of pregnancy in pigs. However, the regulatory mechanism for IL1B receptor expression and the function of IL1B in the uterine endometrium are not well elucidated. In this study, we determined IL1B receptor expression in the uterine endometrium of pigs during pregnancy. IL1B receptor subtypes, IL1 receptor type I (IL1R1) and IL1 receptor accessory protein (IL1RAP) were expressed in the uterine endometrium with the expression being most abundant on Day 12 of pregnancy primarily in the luminal and glandular epithelial cells. Expression of IL1R1 mRNA increased in response to IL1B in a dose-dependent manner, and expression of IL1RAP mRNA increased in response to both IL1B and estradiol, indicating that expression of endometrial IL1B receptors was regulated cooperatively by IL1B and estrogen of conceptus origin. During the peri-implantation period, the porcine uterine endometrium actively synthesizes and secretes prostaglandins (PGs). IL1B increased expression of PTGS1 and PTGS2 genes that are rate-limiting for PG synthesis in the uterine endometrium. Collectively, the results indicated that IL1B regulates ...Continue Reading

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Citations

Nov 26, 2014·Reproductive Biology and Endocrinology : RB&E·Bo Sun JooKyu-Sup Lee
Jul 23, 2014·Asian-Australasian Journal of Animal Sciences·Heewon SeoHakhyun Ka
Jul 28, 2016·Journal of Ovarian Research·Rebeca López-ÚbedaSebastian Canovas
Mar 12, 2013·Journal of Reproductive Immunology·Agata ZmijewskaGenowefa Kotwica
Apr 11, 2017·Molecular Reproduction and Development·Rodney D GeisertThomas E Spencer
Jun 22, 2018·Journal of Animal Science and Biotechnology·Hakhyun KaJisoo Han
Jun 11, 2014·The Journal of Clinical Endocrinology and Metabolism·Kiymet BozaogluJeremy B M Jowett
Jun 20, 2017·Molecular Reproduction and Development·Agnieszka WaclawikAdam J Ziecik
Feb 13, 2019·Asian-Australasian Journal of Animal Sciences·Soohyung LeeHakhyun Ka
Mar 20, 2019·International Journal of Molecular Sciences·Nina SmolinskaTadeusz Kaminski
Jun 25, 2020·Biomolecules·Isabel BarrancoJordi Roca
Sep 3, 2021·Domestic Animal Endocrinology·F R C L Almeida, A L N Alvarenga Dias

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Methods Mentioned

BETA
hysterectomy
electrophoresis
PCR
protein assay

Software Mentioned

SAS
GelPro Analyzer

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