Regulatory mechanisms in bone following mechanical loading.

Gene Regulation and Systems Biology
Sara M Mantila RoosaYunlong Liu

Abstract

Bone responds with increased bone formation to mechanical loading, and the time course of bone formation after initiating mechanical loading is well characterized. However, the regulatory activities governing the loading-dependent changes in gene expression are not well understood. The goal of this study was to identify the time-dependent regulatory mechanisms that governed mechanical loading-induced gene expression in bone using a predictive bioinformatics algorithm. A standard model for bone loading in rodents was employed in which the right forelimb was loaded axially for three minutes per day, while the left forearm served as a non-loaded, contralateral control. Animals were subjected to loading sessions every day, with 24 hours between sessions. Ulnas were sampled at 11 time points, from 4 hours to 32 days after beginning loading. Using a predictive bioinformatics algorithm, we created a linear model of gene expression and identified 44 transcription factor binding motifs and 29 microRNA binding sites that were predicted to regulate gene expression across the time course. Known and novel transcription factor binding motifs were identified throughout the time course, as were several novel microRNA binding sites. These time-...Continue Reading

References

Feb 14, 1998·The Journal of Clinical Investigation·J B KimB M Spiegelman
Nov 13, 1998·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·C H Turner, F M Pavalko
Nov 15, 2001·Obesity Research·M KolehmainenM I Uusitupa
Jan 5, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y F HsiehC H Turner
May 22, 2002·Biochimica Et Biophysica Acta·Hannes OberkoflerWolfgang Patsch
Aug 7, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Alexander G RoblingCharles H Turner
Feb 13, 2003·Nucleic Acids Research·Rafael A IrizarryTerence P Speed
Jan 8, 2004·Annals of Medicine·Donald E Ingber
Sep 24, 2004·The Journal of Biological Chemistry·Daisuke InoueToshio Matsumoto
Jul 19, 2005·Journal of Biomechanics·Jennifer L SchrieferCharles H Turner
May 24, 2006·Genomics·Yunlong LiuHoward J Edenberg
Aug 16, 2006·The Journal of Biological Chemistry·John A RobinsonFrederick J Bex
Aug 26, 2006·Biochemical and Biophysical Research Communications·Astrid LiedertAnita Ignatius
Dec 20, 2007·The Journal of Biological Chemistry·Alexander G RoblingCharles H Turner
Jun 13, 2008·Nature·Brandi N DavisAkiko Hata
Sep 4, 2008·Journal of Cellular Physiology·William KongJin Q Cheng
Oct 18, 2008·Nucleic Acids Research·Qinghua JiangYunlong Liu
Apr 4, 2009·The Journal of Biological Chemistry·Zhaoyong LiJane B Lian
Dec 4, 2009·International Reviews of Immunology·Enikö Sonkoly, Andor Pivarcsi
Jul 27, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Sara M Mantila RoosaCharles H Turner

❮ Previous
Next ❯

Citations

Nov 22, 2016·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Hallie Edmonds

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE22286

Methods Mentioned

BETA
PCR
electrophoresis
Assay

Software Mentioned

Affymetrix GeneChip ® Operating System ( GCOS
MotifModeler
Partek

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.