Regulatory mechanisms of acetylcholine synthesis and release by T cells

Life Sciences
Takeshi FujiiKoichiro Kawashima

Abstract

Muscarinic and nicotinic acetylcholine (ACh) receptors are expressed in immune cells. ACh synthesized by choline acetyltransferase (ChAT) and released in T cells binds to these receptors. Furthermore, we have recently demonstrated the involvement of mediatophore, a homooligomer of a 16-kDa proteolipid subunit of vacuolar H(+)-ATPase, in ACh release from T cells. In this study, we investigated the effects of phorbol 12-myristate 13-acetate (PMA), dibutyryl cAMP (dbcAMP) and FK506, an immunosuppressant calcineurin inhibitor, on lymphocytic cholinergic activity in T cells. We determined the content and release of ACh in human leukemic T cell line MOLT-3 cells using a sensitive and specific radioimmunoassay for ACh. In addition, expression of ChAT mRNA and ChAT activity were investigated using reverse-transcription-polymerase chain reaction and Fonnum method, respectively. Phytohemagglutinin (PHA), a T-cell activator, up-regulated ChAT mRNA expression, synthesis and release of ACh. PMA, a protein kinase C (PKC) activator, and dbcAMP, a protein kinase A (PKA) activator, also increased ChAT activity and ACh synthesis by up-regulating ChAT gene expression. FK506 inhibited PHA-induced up-regulation of ChAT mRNA expression, suggesting t...Continue Reading

References

Feb 1, 1980·Journal of Pharmacological Methods·K KawashimaM Mochizuki
Apr 13, 2000·Pharmacology & Therapeutics·K Kawashima, T Fujii
Sep 9, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Koichiro Kawashima, Takeshi Fujii
Jan 24, 2009·Annals of the New York Academy of Sciences·Yves DunantPaula P Gonçalves
Jan 17, 2012·Journal of Neuroimmunology·Takeshi FujiiKoichiro Kawashima

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Citations

Jun 21, 2014·Immunological Investigations·Zhigang LiuQing Fan
Apr 25, 2015·International Immunopharmacology·Koichiro KawashimaKazuhide Horiguchi
Jul 25, 2012·Annals of the New York Academy of Sciences·Koichiro KawashimaKazuhide Horiguchi
May 22, 2013·ISRN Gastroenterology·Wouter J de Jonge
Dec 6, 2016·International Journal of Molecular Sciences·Maria Di BariAda Maria Tata
Apr 11, 2017·Neuroscience and Biobehavioral Reviews·Bin HanJunwei Hao
Jan 10, 2018·Dermatologic Therapy·Zhun Rui Mok, Hong Liang Tey
Sep 22, 2017·Frontiers in Immunology·Takeshi FujiiKoichiro Kawashima
Nov 12, 2019·Journal of Internal Medicine·M A CoxT W Mak
May 4, 2021·Frontiers in Immunology·Namrita Halder, Girdhari Lal
Jun 1, 2021·Frontiers in Cell and Developmental Biology·Gandhi F Pavón-RomeroLuis M Terán
Jul 3, 2021·International Journal of Molecular Sciences·Masato MashimoTakeshi Fujii
Oct 2, 2021·American Journal of Physiology. Heart and Circulatory Physiology·Adrian H ChesterMagdi H Yacoub

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