Regulatory mutations affecting ornithine decarboxylase activity in Saccharomyces cerevisiae.

Journal of Bacteriology
M S CohnH Tabor

Abstract

We isolated several strains of Saccharomyces cerevisiae containing mutations mapping at a single chromosomal gene (spe10); these strains are defective in the decarboxylation of L-ornithine to form putrescine and consequently do not synthesize spermidine and spermine. The growth of one of these mutants was completely eliminated in a polyamine-deficient medium; the growth rate was restored to normal if putrescine, spermidine, or spermine was added. spe10 is not linked to spe2 (adenosylmethionine decarboxylase) or spe3 (putrescine aminopropyltransferase [spermidine synthease]). spe 10 is probably a regulatory gene rather than the structural gene for ornithine decarboxylase, since we isolated two different mutations which bypassed spe10 mutants; these were spe4, an unliked recessive mutation, and spe40, a dominant mutation linked to spe10. Both spe4 and spe40 mutants exhibited a deficiency of spermidine aminopropyltransferase (spermine synthase), but not of putrescine aminopropyltransferase. This suggests that ornithine decarboxylase activity is negatively controlled by the presence of spermidine aminopropyltransferase.

References

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Citations

Jul 29, 1998·The Plant Journal : for Cell and Molecular Biology·M B WatsonR L Malmberg
Feb 22, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kazuhiro NishimuraKazuei Igarashi
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